%0 Journal Article %A Mohiti Ardakani, javad %A Walker, John %A Qujeq, Durdi %T Serum Factors Induced the Nuclear Location of Annexin V in the Human Osteosarcoma Cell Line (MG-63) %J Iranian Biomedical Journal %V 4 %N 4 %U http://ibj.pasteur.ac.ir/article-1-591-en.html %R %D 2000 %K Annexin V, Calcium, Osteosarcoma cells, %X Calcium-binding proteins play essential roles in the cell. One important class of calcium-binding proteins is the annexin family. This is a family of 13 proteins, which binds to phospholipids in a calcium-dependent manner. Osteosarcoma cell line (MG-63) is a transformed cell that has many characteristics of the differentiated cell, such as a considerable serum dependency in its growth rate. Using specific antibodies against each annexin and immunoflurescence microscopy, the location and relocation of the annexin V was determined by some serum factors. Serum starvation of MG-63 cells increases their doubling time from 24 hours to 4 days. Cells grown in serum contain high levels of annexin V in the cell nucleus whereas in the absence of serum results in loss of nuclear annexin V in about 75% of the cells. Refeeding cells with medium containing 10% serum restore annexin V to the nuclei within 5 hours. Charcoal-treated serum cannot allow annexin V to return to the nucleus. Inhibition of protein synthesis with cycloheximide does not prevent the serum-induced return of annexin V to the nuclei. However, treatment of cells with genistein at a concentration specific for inhibition of tyrosine kinases (200 M) inhibits the relocation of annexin V from cytoplasm to the nucleus. Thus, the cellular location of the annexin V depends on the growth state of the cells. It can be altered by the movement of this protein between the cytosol and the nucleus. %> http://ibj.pasteur.ac.ir/article-1-591-en.pdf %P 117-122 %& 117 %! %9 Full Length/Original Article %L A-10-1-318 %+ %G eng %@ 1028-852X %[ 2000