@ARTICLE{Mahboudi, author = {Davoudi, Noushin and Mahboudi, Fereidoun and Azizi, Mohammad and Adeli, Ahmad and McMaster, Robert. W and }, title = {Introduction of Three Independent Selection Markers in Leishmania}, volume = {7}, number = {1}, abstract ={The pLE2SCX vector was developed for the stable expression of exogenous genes in the protozoan parasite Leishmania. The pLE2SCX construct contains three independent selection markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously cloned Leishmania major hexa-binding protein gene. Selection was based on resistance to the nourseothericin (Ns) which corresponds to sat gene. The two negative selection HSV-TK and CD genes, make the transformed cell sensitive to ganciclovir (GCV) and 5-fluorocytosine (5-FC). The vector was introduced into Leishmania promastigotes by electroporation and maintained as circular form. The selected transfectants were not grown on media with GCV or 5-FC. Using two drug sensitive selectable markers together on a vector is a novel strategy in gene cloning in Leishmania. This stable transfection vector has allowed the permanently expression of several different exogenous genes at the same time in Leishmania }, URL = {http://ibj.pasteur.ac.ir/article-1-547-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-547-en.pdf}, journal = {Iranian Biomedical Journal}, doi = {}, year = {2003} }