Moslem Afsharnezhad, S. Shirin Shahangian, Behnam Rasti, Mohammad Faezi Ghasemi,
Volume 25, Issue 1 (1-2021)
Abstract
Background: This study was devoted to assessing the inhibitory potential of acetone, methanol, and ethanol extracts of Acroptilon repens against disease-associated enzymes, as well as their antioxidant/antibacterial activity and phytochemical composition. Methods: Comparative assessment using various antioxidant evaluation methods, including ferric reducing antioxidant power, scavenging ability on 2,2-diphenyl-1-picrylhydrazyl radical and hydrogen peroxide, and reducing power, indicated that the acetone extract presented the highest antioxidant activity, due to its highest total antioxidant content. Results: The total phenolic content and total flavonoids content of these extracts were 3.44 ± 0.32 mg GAE/g DW and 2.09 ± 0.2 mg QE/g DW, respectively. The hydrodistillation essential oil from A. repens was analyzed by gas chromatography-mass spectroscopy, and 17 compounds were identified. All extracts showed good inhibitory activities against disease-related enzyme acetylcholinesterase and α-amylase, with the lowest IC50 for acetonic extract. Extracts of A. repens exhibited inhibiting activities against the Gram-positive bacteria, with the most effect of acetone extract. Conclusion: Our findings suggest A. repens as a promising source of natural antioxidant, antimicrobial, anti-cholinesterase and anti-amylase agents for the management of oxidative damage, and pharmaceutical, food, and cosmeceutical purposes.
Mercy Mpho Khala, Anneke van der Spoel van Dijk, Polo-Ma-Abiele Hildah Mfengwana,
Volume 28, Issue 5 (9-2024)
Abstract
Background: Tuberculosis (TB) is among the top ten causes of death in South Africa and worldwide and remains a high priority of the World Health Organization. South Africa has a rich tradition for using medicinal plants to treat various diseases, including TB; however, the safety and efficacy of these plants require thorough investigation and confirmation. This study examined the anti-inflammatory and anti-TB activities of three indigenous medicinal plants against two strains of TB.
Methods: Water and methanol root extracts of E. elephantina (Burch.), L. lanceolata, and T. burchellianum were tested in vitro for their activity against Latin American Mediterranean (LAM) and East African Indian (EAI) lineages. Additionally, the phytochemical screening, anti-inflammatory properties, and cytotoxicity activities of these extracts were evaluated.
Results: Aqueous extracts of E. elephantina and T. burchellianum exhibited anti-inflammatory activity at a concentration of 200 μg/ml, while
L. lanceolata demonstrated no activity on macrophage cells. The
E. elephantina extracts showed no cytotoxicity against the Vero cells at high concentration (200 μg/ml); however, both L. lanceolata and T. burchellianum aqueous extracts were found to be cytotoxic at 200 μg/ml. Unfortunately, none of the three plant extracts showed anti-TB activity against LAM and EAI stains.
Conclusion: While the tested extracts lacked direct anti-TB effects, their anti-inflammatory properties and safety profile highlight their potential for adjunctive therapy in TB management or other inflammatory conditions. Further studies are needed to explore the mechanisms and potential clinical applications of these findings.
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