Showing 7 results for Angiogenesis
Piotr Ziolkowski, Beata J.osiecka, Krzysztof Symonowicz1, Piotr Chmielewski, Leszek Latos-Grazynski, Andrzej Bronowicz,
Volume 8, Issue 3 (7-2004)
Abstract
The role of angiogenic molecules, like vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) in tumor angiogenesis was well confirmed. Photodynamic therapy (PDT) action is, to very high degree, based on tumor vasculature damage. Therefore, it seemed to be important to evaluate growth factor receptors after PDT. The extent of receptor expression was studied by immuno-histochemical method. In this study, vascular endothelial growth factor (VEGFR) receptor and fibroblast growth factor (FGFR-1) receptor have been evaluated at different time points after PDT of tumor-bearing BALB/c mice. Two sensitizers: hematoporphyrin derivative (HpD) and 21, 23-dithiaporphyrin (DTP) were given intraperitoneally in doses: 1.25, 2.5 and 5.0 mg/kg followed by light irradiation at total doses: 50 and 100 J/sq.cm 24 hours later. The number of VEGFR and FGFR-1 in control samples did not exceed 40 per one vessel, whereas after PDT, a significant decrease in number of both receptors was observed. No differences between HpD- and DTP-PDT in anti-receptor activities were observed (p<0.001 for VEGFR and p<0.002 for FGFR-1). The observed decrease in VEGFR and FGFR-1 amount confirms that after PDT, some proteins are inactivated and such a decrease may influence PDT effectiveness
Kamran Mansouri, Ali Mostafaei, Manochehr Mirshahi, Hamidreza Mohammadi Motlagh, Ali Maleki, Maryam Keshavarz,
Volume 13, Issue 3 (7-2009)
Abstract
Background: Angiogenesis, the development of new blood vessels, is an important process in tissue development and wound healing, but becomes pathologic when associated with solid tumor growth, proliferative retinopathies, and rheumatoid arthritis. Accurate and reliable qualification of neovascular (angiogenic) response, both in vitro and in vivo, is an essential requirement for the study of new blood vessel growth. The complexity of currently used three-dimensional in vitro angiogenesis systems makes it difficult to approve material in its models. Capillary-like structure occurs on basement membrane components such as collagen and/or laminin, while in other models, CLS formation occurs on transitional matrices such as fibrin. To solve this problem, we were interested in developing an angiogenesis system which allows rapid and reliable quantification of three-dimensional neovessel formation in vitro. Methods: Human bone marrow endothelial cells were seeded on gelatin-coated microcarriers and suspended in a solution of platelet-poor plasma which was induced to polymerize by addition of calcium chloride. In this way, microcarriers were entrapped in three-dimensional coagulated plasma. Results: Within a few hours, endothelial cells begin to leave these supporting microcarries and migrate into the coagulated-plasma matrix and formed CLS within 48-72 hours. Conclusion: We developed a convenient angiogenesis in vitro system which allows reliable quantification of capillary formation in a three-dimensional environment.
Fatemeh Hajighasemi, Sakineh Hajighasemi,
Volume 13, Issue 4 (10-2009)
Abstract
Background: Beta-adrenergic blocking agents have been broadly used for treatment of many cardiovascular diseases such as arterial hypertension and ischemic heart failure. Anti-tumoral, anti-inflammatory and anti-angiogenesis effects of propranolol (a non-selective beta-adrenergic blocker) have been shown. Angiogenesis (replenish of the pre-existing vascular networks) plays a critical role in some pathological conditions such as tumor expansion and metastasis. In this study, we investigated the effects of propranolol on vascular endothelial growth factor (VEGF) production and matrix metalloproteinase-2 (MMP-2) activity (two important angiogenic factors) in human leukemic cell lines in vitro. Methods: Two human leukemic T (Molt-4 and Jurkat) and one monocyte (U937) cell lines were used in this study. The cells were cultured in complete RPMI medium and then incubated with different concentrations of propranolol (0.3-30 µM) in the presence or absence of phorbol myristate acetate (PMA, 25 ng/ml) for 48 hours. The level of VEGF secreted in the cell culture supernatants was measured with enzyme-linked immunosorbent assay kits (R and D systems) and MMP-2 activity in cell-conditioned media was evaluated by gelatin zymography. Results: Propranolol significantly decreased VEGF production and also MMP-2 activity in PMA-activated human leukemic cell lines Molt-4, Jurkat and U937 at 30 µM concentration of the drug compared to untreated control cells (P<0.05). Conclusion: Propranolol might be a useful anti-angiogenic agent in hematopoietic malignancies. Thus, propranolol along with its chronic long-term usage in cardiac problems may have potential implication in treatment of leukemia.
Alireza Garjani, Hassan Rezazadeh, Sina Andalib, Mojtaba Ziaee, Yousef Doustar, Hamid Soraya, Mehraveh Garjani, Arash Khorrami, Mostafa Asadpoor, Nasrin Maleki-Dizaji,
Volume 16, Issue 2 (6-2012)
Abstract
Background: A growing body of preclinical data indicates that statins may possess antineoplastic properties however, some studies have raised the possibility that statins may also have carcinogenic potential. Methods: An air pouch model was used for angiogenesis. Single or multiple applications of croton oil on the back of Swiss albino mice with or without initiation by dimethylbenz(a)antheracene (DMBA) were used to evaluate the skin tumorgenesis, ultrastructural and histological alterations. Results: Atorvastatin (orally, 10 mg/kg/day) produced a significant (P<0.05) reduction in angiogenesis. Concurrent administration of mevalonate reversed the anti-angiogenic effect of atorvastatin. However, local injection of atorvastatin (200 µg) into the pouches induced a significant (P<0.5) increase in angiogenesis that was not reversed by co-administration of mevalonate. The disturbance of cell polarity, inflammatory response, thickness of epidermal layer, and mitotic index induced by croton oil were inhibited markedly and dose-dependently (P<0.001) by pre-treatment with atorvastatin. In spite of the strong anti-inflammatory and anti-proliferative effects of atorvastatin on epidermal cell proliferation, it was identified that the same doses of atorvastatin in DMBA-initiated and croton oil-promoted skin tumorgenesis in mice increased the incidence of tumors and their conversion into malignant carcinoma. Conclusion: The reasons for these discrepancies remain unclear, and could be related to ambivalent effects of atorvastatin on angiogenesis or to specific differences in the experimental conditions. It is suggested that the pro-angiogenic effect of the drug, which could be responsible for promotion of skin tumors, is independent of the 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase inhibition that can be mediated directly by atorvastatin.
Farzaneh Chehelcheraghi, Abolfazl Abbaszadeh, Magid Tavafi,
Volume 22, Issue 5 (9-2018)
Abstract
Background: Skin flap procedures are employed in plastic surgery, but failure can lead to necrosis of the flap. Studies have used bone marrow mesenchymal stem cells (BM-MSCs) to improve flap viability. BM-MSCs and acellular amniotic membrane (AAM) have been introduced as alternatives. The objective of this study was to evaluate the effect of BM-MSCs and AAM on mast cells of random skin flaps (RSF) in rats. Methods: RSFs (80 × 30 mm) were created on 40 rats that were randomly assigned to one of four groups, including (I) AAM, (II) BM-MSCs, (III) BM-MSCs/AAM, and (IV) saline (control). Transplantation was carried out during the procedure (zero day). Flap necrosis was observed on day 7, and skin samples were collected from the transition line of the flap to evaluate the total number and types of mast cells. The development and the total number of mast cells were related to the development of capillaries. Results: The results of one-way ANOVA indicated that there was no statistically significant difference between the mean numbers of mast cell types for different study groups. However, the difference between the total number of mast cells in the study groups was statistically significant (p = 0.001). Conclusion: The present study suggests that the use of AAM/BM-MSCs can improve the total number of mast cells and accelerate the growth of capillaries at the transient site in RSFs in rats.
Maryam Habibi, Farzaneh Chehelcheraghii,
Volume 25, Issue 5 (9-2021)
Abstract
Background: Treatment with bone marrow mesenchymal stem cell (BMMSCs) has anti-inflammatory, tissue regenerative, angiogenic, and immune-stimulating effects. When using as sheets or accumulate, BMMSCs causes the development of neoangiogenesis in damaged skin tissue. Diabetes, a metabolic disorder, can negatively affect many physiological functions, including the process of skin injury repair. This adverse impact may increase the risk of skin surgery. Random skin flap (RSF) is commonly used in reconstructive surgery. The terminal part of the RSF is often affected by necrosis because of impaired blood flow, which is exacerbated in diabetes. This study investigated the effect of stem cells, applied as accumulated or cell sheets, along with RSF surgery on skin capillaries in streptozotocin (STZ)-induced diabetic rats. Methods: Thirty male Wistar rats were divided into three groups (n = 10): diabetes-RSF control, diabetes-RSF local applied stem cells (loc-BMMSCs), diabetes-RSF applied stem cells as accumulated or cell sheets (ac-BMMSCs). Two weeks after the STZ injection, RSF surgery and stem cell therapy (6 × 109) were carried out (day zero). Furthermore, stereological methods were used to investigate the capillary patterns among the groups. Anti-CD31/platelet endothelial cell adhesion molecule-1 immunohistochemistry was also used for further confirmation of changes in capillary parameters. Results: The results demonstrated that capillaries were protected by MSC sheets in the flap tissue, and the thickness of the epidermal layer was improved, indicationg the possible beneficial effects of MSC sheets on diabetic wound treatment. Conclusion: Stem cells, as ac-BMMSCs, may decrease the levels of wound healing complications in diabetes and can be considered as a cell therapy option in such conditions.
Fereshteh Vaziri Nezamdoust, Masoumeh Gahramanpoor, Yaghub Pazhang, Reza Rahbarghazi,
Volume 28, Issue 0 (12-2024)
Abstract
Introduction: Colorectal cancers have been considered devastating pathological conditions with high prevalence rates in developing and developed countries. Finding effective oncostatic compounds with fewer side effects is central to the debate. Phytocompounds with varied biological effects have been used to prevent and treat different tumors. In the present study, we investigated the tumoricidal effects of resveratrol on human colorectal HT-29 cells.
Methods and Materials: Cells were treated with different concentrations of resveratrol (25, 50, and 100 µM) for 48 hours. The survival rate, expression, and protein levels of angiopoietin (Ang)-1 and -2 were monitored using MTT, real-time PCR, and Western blotting.
Result: Data indicated the reduction of survival rate in HT-29 cells in a dose-dependent manner after being exposed to 25, 50, and 100 µM of resveratrol compared to the control group (p = 0.05). Based on real-time PCR and Western blot analyses, increasing doses of resveratrol can reduce the expression and protein levels of Ang-1 and -2 in a dose-dependent manner (p = 0.05).
Conclusion and Discussion: Resveratrol can reduce the angiogenesis properties of human colorectal HT-29 cells by suppressing Ang-1 and -2 in vitro conditions.
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