Showing 12 results for Yari
Bahram Haghighi, Mohamad Yari, Shahraz Tori,
Volume 4, Issue 1 (1-2000)
Abstract
The mechanism by which bi-and trivalent cations affect human liver phosphatidatephosphohydrolase (PAP) activity was investigated. Bivalent cations up to 1 mM increased PAP activity whereas at higher concentrations the activity of the enzyme decreased. The stimulatory concentration for trivalent cations such as Al3+ and Cr3+, however, was much lower being 2 m M and 1 m M, respectively. All cations affecting PAP activity were also able to induce phase transition of phosphatidate from lamellar (La) to inverted hexagonal (HII) form. The rate of La-HII transition was different for each cation. At 100 mM concentration of Mg2+ only 26% of the original phosphatidate remained in La form and for other cations tested ranged from 14.5% to 76%. The phase transition was blocked by EDTA. Magnesium from 0.8 to 1.5 mM concentration raised PAP activity (3-fold) with La form of substrate but not with the HII phase. Monovalent cations such as Na+ and K+ neither affected enzyme activity nor substrate configuration. These data suggest that cation-induced PAP activation is not as a result of cation-protein interaction, but is due to formation of a suitable substrate configuration for the enzyme catalysis during phosphatidate phase transition. It appears that the real substrate configuration for PAP activity is situated between La and HII phases.
Shamsi Yari, Davoud Nouri Inanlou, Fatemeh Yari, Maryam Saleh, Behrokh Farahmand, Azim Akbarzadeh,
Volume 6, Issue 1 (1-2002)
Abstract
In this study, mutant forms of Bacillus thuringiensis spp. israelensis (H14) were produced. These mutants were identified when the cells were cultured on chloramphenicol plates and stained with crystal violet. Protoplasts of the mutants were isolated by enzymatic digestion (lysozyme) of the cell walls at the presence of an osmotic stabilizer. The protoplasts were induced to fuse to each other in the presence of PEG 6000. The frequency of regeneration and recombination was 80% and 2´10-4, respectively. In order to survey the effect of protoplast fusion on production of toxin, anti-serum against pure toxin was raised in rabbit and was used in single radial immunodiffusion. The comparison of d-endotoxin concentration between B. thuringiensis fusion and the wild type strains showed that B. thuringiensis fusion has 1.48 time more toxin than wild type.
Hamdollah Delaviz, Mohammad Taghi Joghataie, Mehdi Mehdizadeh, Mehrdad Bakhtiyari, Maliheh Nobakht, Samideh Khoei,
Volume 12, Issue 4 (10-2008)
Abstract
Background: Olfactory ensheathing glia (OEG) has been shown to have a neuroprotective effect after being transplanted in rats with spinal cord injury. This study was conducted to determine the possible beneficial results of olfactory mucosa transplantation (OMT) which is a source of OEG on functional recovery and axonal regeneration after transection of the sciatic nerve. Methods: In this study, 36 adult female Sprague-Dawley rats were used. The sciatic nerve was transected in 24 rats and immediately repaired by sciatic-sciatic anastomosis, and randomly divided equally into two groups. The experimental group received the OMT at the transected site and the control group received the respiratory mucosa transplant. In another twelve rats as sham-operated animals, the sciatic nerve was exposed but no transection was made. DiI retrograde tracing was injected in the gastrocnemius muscle two months after surgery to allow visualization of the extent of axonal regeneration. Functional recovery was also assessed at 15, 30, 45 and 60 days after surgery using walking track analysis and sciatic function index (SFI) calculations. Results: The total number of DiI labeled motorneurones in the ventral horn (L4-L6) and the SFI scores were significantly higher in the group of rats that received olfactory mucosa rather than respiratory mucosa. Conclusions: The outcome indicates that olfactory mucosa is a useful treatment to improve nerve regeneration in mammals with peripheral nerve injury.
Bahram Kazemi, Farideh Tohidi, Mojgan Bandehpour, Fatemeh Yarian,
Volume 14, Issue 3 (11-2010)
Abstract
Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pteridine moiety from guanine triphosphate. Therefore, it must produce pteridine using PTR1, an essential part of the salvage pathway that reduces oxidized pteridines. Thus, PTR1 is a good drug-target candidate for anti-Leishmania chemotherapy. The aim of this study was the cloning, expression, and enzymatic assay of the ptr1 gene from Iranian lizard Leishmania as a model for further studies on Leishmania. Methods: Promastigote DNA was extracted from the Iranian lizard Leishmania, and the ptr1 gene was amplified using specific primers. The PCR product was cloned, transformed into Escherichia coli strain JM109, and expressed. The recombinant protein (PTR1 enzyme) was then purified and assayed. Results: ptr1 gene was successfully amplified and cloned into expression vector. Recombinant protein (PTR1 enzyme) was purified using affinity chromatography and confirmed by Western-blot and dot blot using anti-Leishmania major PTR1 antibody and anti-T7 tag monoclonal antibody, respectively. The enzymatic assay was confirmed as PTR1 witch performed using 6-biopterin as a substrate and nicotinamide adenine dinucleotide phosphate as a coenzyme. Conclusion: Iranian lizard Leishmania ptr1 was expressed and enzymatic assay was performed successfully.
Abbas Ebrahimi-Kalan, Jafar Soleimani Rad, Laya Kafami, Daryoush Mohammadnejad, Mehryar Habibi Roudkenar, Amir Afshin Khaki, Zeynab Aliyari Serej, Amaneh Mohammadi Roushandeh,
Volume 18, Issue 4 (10-2014)
Abstract
Notice of the Full Retraction of the Above-Mentioned Article
Due to the redundant information presented in this paper and the paper published in the Iran Red Crescent Journal: "MS14, a Marine Herbal Medicine, an Immunosuppressive Drug in Experimental Autoimmune Encephalomyelitis. Iran Red Crescent Med J. 2014 July; 16(7): e16956, the editor/publisher primarily declared a notice of "partial retraction" (dated 26 August, 2020). But since the amount of redundant information was later found very significant (Figure-1 and the graph in Figure-2) and its omission would not leave a credible paper, IBJ was obliged to issue a notice of full retraction on 1 November 2020. The authors are not in agreement with this decision.
Notice of the Partial Retraction of the Above-Mentioned Article
The authors would like to retract Figure 1 from the paper entitled: “MS14 Down-regulates Lipocalin2 Expression in Spinal Cord Tissue in an Animal Model of Multiple Sclerosis in female C57BL/6”. The reason for this partial retraction is the duplicity of this Figure in the addressed paper and the paper entitled: “MS14, a Marine Herbal Medicine, an Immunosuppressive Drug in Experimental Autoimmune Encephalomyelitis” (Iran Red Crescent Med J, 2014 July; 16(7): e16956). The duplicity was inevitable, as the data was needed for the presentation of the rest of the data in the former paper, whereas the latter paper was not then published. However, considering both papers are published at this time, we would like to retract Figure 1 from the above-addressed paper.
Azar Babakhani, Malihe Nobakht, Hamidreza Pazoki Torodi, Mostafa Dahmardehei, Paria Hashemi, Javad Mohajer Ansari, Parisa Ramhormozi, Abazar Yari, Fatemeh Heidari,
Volume 24, Issue 2 (3-2020)
Abstract
Background: The recent improvements in wound healing have led to new strategies in regenerative medicine. Burn wound healing is an important issue in skin regeneration and has multiple indications for stem cell therapy. Hair follicle stem cells (HFSCs) are a highly promising source of stem cells for healing use, as these cells are accessible, active and pluripotent adult stem cells. Methods: HFSCs of the rat whisker were isolated, cultured, and labeled with DiI. Flow cytometry method was used to detect special markers of HFSCs. Deep partial-thickness burn wound was created, and labeled HFSCs were injected around the wound bed. Wound closure was recorded via digital photographs. The inflicted rats were sacrificed at 3, 7, or 14 days post burn and used for subsequent histological and tensiometry analysis. Results: Our results indicated that HFSCs were positive for Nestin and CD34 markers, but negative for Kr15. Morphological and histological photographs revealed that wound closure rate was accelerated in stem cell-treated group compared with other groups. In addition, faster re-epithelialization and collagen deposition were observed. The immunohistochemical analysis suggested that CD31 expression and vascular density enhanced in the stem cell-treated group. Further, tissue tensile strength increased in HFSCs-treated rats in comparison to the control group. Conclusion: The present study demonstrates that HFSC could accelerate burn wound healing as well as tensile strength in rats.
Abbas Ebrahimi-Kalan , Jafar Soleimani Rad, Laya Kafami , Daryoush Mohammadnejad, Mehryar Habibi Roudkenar, Amir Afshin Khaki, Zeynab Aliyari, Amaneh Mohammadi Roushandeh,
Volume 24, Issue 6 (11-2020)
Abstract
Notice of the Retraction of the Above-Mentioned Article:
The publisher would like to retract the above-mentioned article due to the partial duplicity of the therein presented data with those of another article, entitled: “MS14, a Marine Herbal Medicine, an Immunosuppressive Drug in Experimental Autoimmune Encephalomyelitis” (Iran Red Crescent Med J, 2014 July; 16(7): e16956).
Venus Zafari, Milad Asadi, Nasim Bakhtiyari, Mahsa Sadeghzadeh, Majid Khalili, Habib Zarredar, Soghra Bornehdeli, Ensiyeh Seyedrezazadeh,
Volume 26, Issue 3 (5-2022)
Abstract
Background: Let-7f has essential impacts on biological processes; however, its biological and molecular functions in lung cancer pathogenesis have yet been remained unclear. We aimed to investigate the expression level of let-7f and its candidate target genes both in lung cancer tissues and A549 cell line.
Methods: Bioinformatics databases were first used to select candidate target genes of let-7f. Then the relative gene and protein expressions of let-7f and its target genes, including HMGA2, ARID3B, SMARCAD1, and FZD3, were measured in lung tissues of Non-Small Cell Lung Cancer (NSCLC) patients and A549 cell line using quantitative real-time PCR and Western blotting. The electroporation method was used to transfect A549 cells with let-7f mimic and microRNA inhibitor. The impact of let-7f transfection on the viability of A549 cells was assessed using MTT assay. The expression data of studied genes were analyzed statistically
Results: Results indicated significant downregulated expression level of let-7f-5p (p = 0.0013) and upregulated level of the HMGA2 and FZD3 in NSCLC cases (p < 0.05). In A549 cells, after transfection with let-7f mimic, the expression of both mRNA and protein levels of HMGA2, ARID3B, SMARCAD1, and FZD3 decreased. Also, the overexpression of let-7f significantly inhibited the A549 cell proliferation and viability (p = 0.017).
Conclusion: Our findings exhibited the high value of let-7f and HMGA2 as biomarkers for NSCLC. The let-7f, as a major tumor suppressor regulatory factor via direct targeting genes (e.g. HMGA2), inhibits lung cancer cell viability and proliferation and could serve as a marker for the early diagnostic of NSCLC.
Raziyeh Najafloo, Rana Imani, Mahla Behyari, Shirin Nour,
Volume 27, Issue 2 (3-2023)
Abstract
Background: Infection is one of the significant challenges in medical implant-related surgeries. Despite systemic antibiotic therapies, bacterial growth after implantation may cause implant failure. Nowadays, unlike the systemic therapy, local controlled release of antibiotic agents is considered an effective approach for the prevention of implant-related infections. The present study aimed to develop a niosomal nanocarrier incorporated into fibroin films for local and continuous delivery of thymol, a natural plant-derived antimicrobial agent for preventing infections caused by implant-related.
Methods: Niosomes containing thymol were prepared by thin-film hydration technique. Thymol sustained release from the prepared films was assessed for 14 days. Antibacterial activities of the synthesized films were also evaluated by the agar diffusion technique against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus.
Results: The release behavior from the niosomal thymol films showed a sustained manner, in which the amount of the released thymol reached 40% after 14 days. The films containing thymol with and without niosome showed a significant viability against L929 fibroblast cells compared to other groups after 24 and 48 h, using MTT assay. Also, samples exhibited potent antibacterial activity against Gram-negative and Gram-positive bacteria.
Conclusion: The results of this study demonstrate that the niosomal thymol-loaded fibroin film is a promising candidate for the controlled release of thymol and prevention of implant-related infection.
Rahim Khodayari Zarnagh, Sanaz Borzoeipour, Mahtab Nikpour Dehkordi,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Population and related discussions are among the complexities of human societies and constitute one of the most important economic and social components of any planning and policymaking process worldwide. Population policies, which are subject to change, play a crucial role in the economic and social development of society. The total fertility rate is one of the most significant indicators of demography. Given the declining trend of this rate below the replacement level, there is an urgent need to adopt effective and efficient policies. Therefore, this study explores the future of policies aimed at increasing the fertility rate through scenario writing methods. By providing a clear and comprehensive understanding of the factors that shape the future, this research assists policymakers in designing efficient and effective interventions to enhance the fertility rate.
Methods and Materials: This study represents a form of future research that employs approach with a 10-year horizon. The scenario writing process consisted of two main components: the first component involved identifying scenario logic through five initial steps, including focal issues, key factors, external ends, critical uncertainties, and scenario logic. The second component, which included secondary steps such as scenarios, implications, and options, concentrated on developing the actual narrative, solutions, and policy options for the scenarios.
Results: Six main categories identified a total of 106 drivers. Most drivers belonged to the social category, whereas the technology category had the fewest drivers. After eliminating less essential factors, validating trends, and categorizing key uncertainties, we selected four scenarios based on critical uncertainties (social and political developments and economic situation), which significantly impacted the future fertility rate. We ultimately determined future scenarios of the fertility rate using the Cross-Impact Matrix and the Scenario Wizard software, identifying desirable scenarios. We listed various scenarios based on the index score of the total impacts and the inconsistency coefficient. We wrote a related story and narrative for each scenario and selected a relevant and appropriate name.
Conclusion and Discussion: According to the findings of the current study, social, technological, environmental, economic, political, and health factors significantly impact fertility. Additionally, key drivers within each category were identified and discussed, which have been corroborated by various studies worldwide. The primary factors contributing to the decline in fertility rates include the increase in women's education levels and employment opportunities, which should be carefully considered in policymaking. Government policies that support women's education and employment—such as extending parental leave, enhancing maternity leave, and providing financial incentives—should also aim to promote higher fertility rates.
Hamidreza Golian, Mohammad Amin Ghezel, Nooshin Roudbari, Morteza Eskandari, Mehrdad Hosein-Zadeh, Neda Asl Estiyari, Amir Arshiya Mohammadi Vala, Shilan Nasri,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Formaldehyde (CH2O) poisoning causes widespread neuronal death in the central nervous system by inducing oxidative stress. This study aimed to evaluate the therapeutic effects of propofol and the antioxidant CoQ10 on the neuronal density of the entorhinal cortex, hippocampal pyramidal cells, and oxidative stress factors in the fetal forebrain following formaldehyde poisoning.
Methods and Materials: Thirty-five pregnant Wistar rats were randomly divided into seven control groups: formaldehyde + saline, formaldehyde + propofol 10 mg/kg, formaldehyde + propofol 20 mg/kg, formaldehyde + propofol 40 mg/kg, formaldehyde + CoQ10 50 mg/kg, and formaldehyde + propofol 40 mg/kg + CoQ10 50 mg/kg. To induce formaldehyde poisoning, on the 14th day of pregnancy, formaldehyde (20 mg/kg body weight) was injected intraperitoneally into pregnant rats. From the 12th to the 18th day of pregnancy, the treatment groups received propofol with different doses and coenzyme Q10 via the gavage method. After cesarean delivery of the fetuses on the 21st day of pregnancy, the neuronal density of the entorhinal cortex, CA1 and CA3 areas of the hippocampus, and the tissue levels of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GPX) in the forebrain of the fetuses were evaluated using the ELISA method.
Results: The results showed a significant increase in the activity of CAT, GPX, and SOD enzymes and a significant decrease in MDA levels in the forebrain of fetuses receiving propofol plus CoQ10 compared to the formaldehyde + saline group. Additionally, an increase in the density of neurons in the entorhinal cortex and CA1/CA3 areas of the hippocampus was observed in the groups receiving propofol + coenzyme Q10 compared to the formaldehyde + saline group.
Conclusion and Discussion: Prenatal formaldehyde poisoning, by inducing oxidative stress in the fetal forebrain, caused damage to the entorhinal cortex and hippocampus of the rat fetal brain. On the other hand, propofol improved neuronal damage in these regions of the fetal brain. Propofol can be used as an efficient and effective drug in the treatment of formaldehyde poisoning and brain surgeries. It can prevent the spread of tissue damage to nearby tissues and facilitate the treatment process by using it during the complete treatment of the poisoned individual.
Ziaeddin Bonyadi, Mohammad Reza Yari,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Machine learning (ML), artificial intelligence (AI), and innovative technology are increasingly applied in water treatment and monitoring to optimize processes and address complex challenges. This review article provides an overview of the current applications and advancements in AI, ML, and innovative technology within the water industry.
Search Strategy: PubMed, Web of Science, Scopus, and Google Scholar databases were systematically reviewed using the keywords "machine learning," "artificial intelligence," "smart technology," "water treatment," and "water monitoring." The literature search was limited to include peer-reviewed articles written in English, primarily from 2018 to 2023.
Results: AI methods have been demonstrated to control chlorination effectively. At the same time, ML models are effective in modeling Disinfection By-Product (DBP) concentrations and important parameters for adsorption and membrane filtration processes. The results are often evaluated using various statistical measures, including the coefficient of correlation (R), the coefficient of determination (R2), the mean average error (MAE), the mean square error (MSE), the root mean square error (RMSE), and relative error (RE). ML for adsorption processes has the potential to support operator decisions. Other models that researchers have studied with notable success include Adaptive Neuro-Fuzzy Inference Systems (ANFIS), Support Vector Machine (SVM), and Random Forest (RF). On average, artificial neural network (ANN), support vector machine (SVM), and RF ML models performed well, typically achieving R2 values greater than 0.9 and sometimes exceeding 0.99.
Conclusion and Discussion: The review highlights the increasing adoption of AI, ML, and innovative technology in the water industry, with significant investments expected to drive further advancements in the coming years. While these technologies have successfully optimized water treatment processes and monitored natural systems, challenges such as data quality, model interpretability, and scalability must be addressed to facilitate widespread implementation. Further research and innovation are needed to overcome these challenges and unlock the full potential of AI, ML, and innovative water treatment and monitoring technology.
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