Showing 5 results for Qujeq
Durdi Qujeq,
Volume 3, Issue 1 (1-1999)
Abstract
To improve the specificity of biochemical markers of myocardial infarction (MI) a method to measure cardiac troponin-I (CTn-I) was developed. CTn-I is a protein unique to cardiac muscle and is released after MI. Consecutive 150 patients admitted to the coronary care unit was studied. Value of CTn-I was determined in all samples. CTn-I concentration in the MI patients group was increased compared to that in the control [18.7 +/- 0.13 (mean +/- SD, n = 100 ) vs. 5.31 +/- 0.13, n = 50] µg/l. Measurement of CTn-I accurately detects MI in patients and should facilitate the diagnosis and management of such patients.
Razieh Yazdanparast, Durdi Qujeq,
Volume 3, Issue 1 (1-1999)
Abstract
The activity of one of the metabolizing enzymes of - aminobutyric acid, (GABA), was determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-8) and caerulein (CLN). The activity of this rate-limiting enzyme, L-glutamic acid decarboxylase, (GAD), did not change thirty minutes after peripheral injections of either CCK-8 or CLN in doses of 50g/kg body weight. However, the activity of GAD started to increase 24 hours after injection and continued to increase by 15 to 17%, five days after injections. Based on the in vitro models, it has been shown that there are no direct interactions between CCK-8 and CLN and the enzyme GAD. These data probably support indirect but specific interactions between CCK-8, CLN and the enzyme GAD.
Durdi Qujeq, Gholamreza Ataei,
Volume 4, Issue 2 (3-2000)
Abstract
Because the ingestion of some types of dietary fibers has been shown to influence on the lipid and lipoprotein levels, it is possible that chitosan influences on lipid metabolism. In the present study, the effects of chitosan on the serum, liver lipid and lipoprotein concentrations in rat were investigated. Serum lipid level in the treatment groups were decreased compared to that of the control, cholesterol level [128.65 +/- 2.58 (mean +/- SD, n = 72) vs. 173.67 +/- 3.62, p<0.05] mg/dl, triglyceride level [62.83 +/- 2.73 (mean +/- SD) vs. 93.62 +/- 2.64, p<0.05] mg/dl, and low density lipoprotein-cholesterol level [108.35 +/- 2.41(mean +/- SD) vs. 156.49 +/- 2.37, p<0.05] mg/dl. In the chitosan treatment group, high density lipoprotein-cholesterol level was increased as compared to the control [187.39 +/- 2.74 (mean +/- SD) vs. 163.54 +/- 2.83, p<0.05] mg/dl. This work showed that the addition of chitosan to the diet of the rats significantly lowered the liver lipid in the treatment groups compared to that of the control, cholesterol level [31.53 +/- 1.26(mean +/- SD) vs. 64.42 +/- 2.38, p<0.05] mg/g, and triglyceride level [38.46 +/- 2.64 (mean +/- SD) vs. 53.24 +/- 2.45, p<0.05] mg/g. When chitosan fed at the 5% level, concentration of the serum cholesterol was reduced by 25.92% and triglyceride by 32.89%. The data presented here indicated possible usefulness of chitosan for the treatment of hyperlipidemia.
Javad Mohiti Ardakani, John Walker, Durdi Qujeq,
Volume 4, Issue 4 (10-2000)
Abstract
Calcium-binding proteins play essential roles in the cell. One important class of calcium-binding proteins is the annexin family. This is a family of 13 proteins, which binds to phospholipids in a calcium-dependent manner. Osteosarcoma cell line (MG-63) is a transformed cell that has many characteristics of the differentiated cell, such as a considerable serum dependency in its growth rate. Using specific antibodies against each annexin and immunoflurescence microscopy, the location and relocation of the annexin V was determined by some serum factors. Serum starvation of MG-63 cells increases their doubling time from 24 hours to 4 days. Cells grown in serum contain high levels of annexin V in the cell nucleus whereas in the absence of serum results in loss of nuclear annexin V in about 75% of the cells. Refeeding cells with medium containing 10% serum restore annexin V to the nuclei within 5 hours. Charcoal-treated serum cannot allow annexin V to return to the nucleus. Inhibition of protein synthesis with cycloheximide does not prevent the serum-induced return of annexin V to the nuclei. However, treatment of cells with genistein at a concentration specific for inhibition of tyrosine kinases (200 M) inhibits the relocation of annexin V from cytoplasm to the nucleus. Thus, the cellular location of the annexin V depends on the growth state of the cells. It can be altered by the movement of this protein between the cytosol and the nucleus.
Durdi Qujeq, Laia Hossini, M. Taghi Salehi Omran,
Volume 5, Issue 2 (3-2001)
Abstract
Recent studies have documented the importance of lipids and lipoproteins as determinants of serum total homocysteine concentrations in persons with myocardial infarction, and in healthy subjects. Associations between various biological systems and total homocysteine have also been reported. However, some of these associations are poorly understood. The purpose of our study was to measure serum total homocysteine concentrations in men and women who were selected for health and myocardial infarction to determine the relations of triglycerides, cholesterol and diastolic blood pressure. The study group consisted of 136 patients, 77 male and 59 females, aged 39-73 (mean 46.75 3.91). The control group consisted of 145 normal volunteers, 81 males and 64 females, aged 31-63 (mean 41.72 6.89). Serum total homocysteine was determined by gas chromatograph. Serum cholesterol and triglyceride were measured enzymatically on a spectrophotometer. Our results showed that serum total homocysteine concentrations were significantly positive correlated with cholesterol, triglyceride and diastolic blood pressure.
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