Showing 5 results for Khodadadi
Mohammad H. Pipelzadeh, Abolghasem Jouyban, Ali Khodadadi, Ian L. Naylor,
Volume 5, Issue 2 (3-2001)
Abstract
The absence of a universally accepted mathematical model for assessment of the effectiveness of drugs in the process of wound healing is one of the problems the health professionals have been faced. In this study, an “empirical” mathematical model is presented to evaluate its reliability from an experimental viewpoint and from a comparative aspect with previous models used to study wound healing, namely the log and square root models. The excisional wounds inflicted were based on suggestion of Cross et al., 1995, using a variety of drugs. The drugs influence the wound healing process such as indomethacin, mepyramine, dexamethasone, acetylsalicylic acid and prednisolone. The proposed model is based on a quadratic equation having two constants: and. The -/ ratio and the area-under the curve (AUC) are suggested as being valuable and reliable quantitative values. Also, having more precision in predicting and for expressing the effectiveness of drugs on the wound healing process than the two previous models.
Maryam Yadegari, Mahmoud Orazizadeh, Mahmoud Hashemitabar, Ali Khodadadi,
Volume 15, Issue 4 (10-2011)
Abstract
Background: Current treatments for joint diseases are moderately successful, but unfortunately are associated with significant side effects. This study was undertaken to investigate the combination effects of IL-4 and prednisolone on tissue characteristics and production of matrix metalloproteinase-1(MMP-1) in IL-lα-treated bovine nasal cartilage (BNC) explants. Methods: BNC explants were cultured in DMEM with IL-lα (10 ng/ml), IL-4 (50 ng/ml) and prednisolone (1 or 1,000 nM) at the same time for 28 days. At days 3, 7, 14, 21and 28, the media were collected and replaced with fresh media, and the removed media were stored at -20°C. The alterations of tissue characteristics were assessed by using histology techniques. Western-blot method was used to determine the effects of IL-4 and prednisolone combination on MMP-1 production. The cell viability was evaluated by using lactate dehydrogenase assay test. Results: In the presence of IL-lα alone, most chondrocytes were transformed into fibroblast-like morphology with pyknotic nuclei at day 28. In addition, a clear band of MMP-1 and extracellular matrix (ECM) degradation were observed. In combination of IL-4 and prednisolone, chondrocytes preserved their ordinary normal features. MMP-1 band formation was completely inhibited and ECM absolutely showed normal characteristics. IL-4 and prednisolone did not show cytotoxicity effects on BNC explant culture. Conclusion: This combination can strongly preserve cartilage from degradation features and the data possibly suggest that the combination of IL-4 and prednisolone could be a candidate for alternative therapy in joint diseases.
Maryam Yadegari, Mahmoud Orazizadeh, Mahmoud Hashemitabar, Ali Khodadadi,
Volume 17, Issue 4 (10-2013)
Abstract
Background: Previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. In the current study, effects of IL-4 against morphological changes and tissue degradation induced by IL-1α on bovine nasal cartilage (BNC) explants were investigated. Methods: Fresh BNC samples were prepared from a slaughterhouse under sterile conditions. BNC explants culture was treated with both IL-lα (10 ng/ml) and IL-4 (50 ng/ml) at the same time for 28 days. The morphological characteristics of explants were assessed by using histology techniques and invert microscopy. Matrix metalloproteinase-1 (MMP-1) production was assessed within different days by using Western blotting. Results: IL-lα induced prominent cartilage morphology degradation. The pro and active form of MMP-1 band substantially increased at day 21 of culture. In the presence of both IL-lα and IL-4, chondrocytes preserved their ordinary normal phenotype with intact extracellular matrix. In addition, a significant reduction in pro-MMP-1and inhibition of active MMP-1 was seen. Conclusion: In conclusion, IL-4 could be regarded as a potential candidate in cartilage protecting against the degradation changes of IL-lα. It seems that the preservation effect of IL-4 is associated with significant reduction of MMP-1.
Farhad Pajang, Sanaz Kazemipour, Ali Khodadadi,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Antibiotics are among the most prescribed drugs today, and unfortunately, they are often used in a self-medicated manner. This problem can lead to antibiotic resistance, which is predicted to cause 10 million deaths by 2050, according to the World Health Organization (WHO). Self-medication with antibiotics (SMA) can also cause autoimmune and hypersensitivity reactions due to the imbalance of normal flora. This study aimed to determine the prevalence of SMA in children under 12 years old with common cold symptoms who visited the Isfahan Shariati Hospital clinic during the second half of 2022.
Methods and Materials: This analytical cross-sectional study was conducted from September 2022 to February 2023 at the Isfahan Shariati Hospital. The study population included mothers with children under 12 years old whose common Cold was confirmed by a pediatrician. A total of 433 children were surveyed, of whom 71 self-medicated with antibiotics. Data were collected using a checklist and analyzed using SPSS software version 24. Any use of antibiotics without a prescription was counted as SMA.
Results: The results showed that 71 children (16.4%) had used antibiotics in a self-medicated manner during their colds, with 6 of them (8.5%) using the antibiotics correctly despite self-medication. Additionally, 59 mothers (83%) had not read the drug leaflet. Amoxicillin was the most commonly used antibiotic (20%). The primary reason for SMA was that a previous doctor had prescribed the same antibiotic, and the second reason was the mothers’ belief in the antiviral effects of antibiotics. The average age of mothers who self-medicated with antibiotics was significantly higher than those who did not (p = 0.05). The prevalence of SMA was higher in stay-at-home mothers than in working mothers (p = 0.05).
Conclusion and Discussion: The prevalence of 16.4% is moderate compared to the community setting of Iran, but it is still high. Educational interventions through media and social networks are suggested to raise public awareness regarding the proper use of antibiotics and the dangers of self-medication in both children and adults. Educating students can also help prevent the spread of viral diseases and reduce antibiotic consumption.
Homa Khodadadi, Amirhossein Ebrahimi, Fatemeh Jalali, Gholamreza Askari, Zahra Hajhashemy,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Hypertension is considered a global issue and associated with cardiovascular disease, stroke, and kidney failure. One of the critical risk factors for hypertension is nutrient status; selenium may affect this disorder. Studies have investigated the relationship between blood selenium and hypertension, but their results are inconsistent. We aimed to examine this relationship through a systematic review and dose-response analysis.
Search Strategy: Medline (PubMed), Scopus, Web of Science, and Google Scholar were systematically searched, by using the concepts of selenium, hypertension, and related keywords, up to December 2023, without any restrictions in publication year or language. Observational studies were included that reported blood selenium as exposure and hypertension as outcome in adults, along with relative risks (RRs), odds ratios, or hazard ratios (HRs) and 95% confidence intervals (CIs) for the relation of blood selenium and hypertension. Analyses were conducted using the random effect model. Sensitivity analysis, meta-regression and dose-response analysis were performed. All analyses were conducted using STATA version 14.0.
Results: Overall, 28,312 participants in 17 observational studies were included. The highest versus the lowest levels of circulating selenium were related to 15% higher odds of hypertension; this association was insignificant (95% CI: 0.99-1.33). Subgroup analysis based on sex indicated that highest versus the lowest blood selenium was related to a 36% higher risk of hypertension in women (95% CI: 1.03-1.81). There was no significant association observed in men (RR: 1.23; 95% CI: 0.79-1.90) or when both genders were considered together (RR: 1.02; 95% CI: 0.86-1.21). Linear dose-response analysis revealed no significant relationship between each 50 µg/L increment in blood selenium and hypertension (RR:0.99; 95% CI: 0.94-1.04). To obtain generalizable findings for the adult population, the analyses were limited to representative studies that used a random sampling method for data selection. Similar results were observed in meta-analyses and dose-response analyses of representative studies.
Conclusion and Discussion: The association between blood selenium and hypertension was found to be insignificant in both the general and representative adult populations. However, in certain subgroups, a relationship was observed, potentially due to the toxic effects of selenium. Further prospective studies are necessary to verify the relationship and to elucidate the underlying mechanisms.
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