Showing 3 results for Karbalaie
Marzieh Derakhshan-Horeh, Farid Abolhassani, Farnoosh Jafarpour, Ashraf Moini, Khadijeh Karbalaie, Sayyed Morteza Hosseini, Somayyeh Ostadhosseini, Mohammad Hossein Nasr-Esfahani*,
Volume 21, Issue 1 (1-2017)
Abstract
Backgrund: Imprinted genes are a unique subset of few genes, which have been differentially methylated region (DMR) in a parental origin-dependent manner during gametogenesis, and these genes are highly protected during pre-implantation epigenetic reprogramming. Several studies heve shown that the particular vulnerability of imprinting genes during suboptimal pre- and peri-conception microenvironments often occur by assisted reproduction techniques (ART). This study investigated the methylation status of H19/IGF2 DMR at high-quality expanding/expanded human blastocysts donated by healthy individuals to evaluate the risks linked to ART. Method: Methylation levels of H19/IGF2 DMR were analyzed by bisulfite conversion and sequencing at 18 CpG sites (CpGs) located in this region. Result: Results showed that the overall percentage of methylated CpGs and the proportion of hyper-methylated clones of H19/IGF2 DMR in analyzed blastocysts were 37.85±4.87% and 43.75±5.1%, respectively. For validation of our technique, the corresponding methylation levels of peripheral human lymphocytes were defined (49.52±1.86% and 50%, respectively). Conclusion: Considering the absence of in vivo produced human embryos, it is not possible to conclude that the methylation found in H19/IGF2 DMR is actually normal or abnormal. Regarding the possible risks associated with ART, the procedures should be optimized in order to at least reduce some of the epigenetic risks.
Alireza Tabibzadeh, Maryam Esghaei, Farhad Zamani, Saber Soltani, Parastoo Yousefi, Mohammad Hadi Karbalaie Niya,
Volume 25, Issue 4 (7-2021)
Abstract
To the editor:
Since the initial onset of the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic from Wuhan, China in December 2019, the virus is now spread all around the world, and the disease is named COVID-19. Based on the WHO report on April 18, 2021, there are now more than 140 million confirmed cases and three millions deaths due to this disease. The virus pathogenesis, clinical features, and therapeutic approaches have comprehensively been reviewed. In addition, it has been indicated that patients' medical background and the age factor are associated with the mortality rate. Regardless of the clinical presentations and the disease mortality, the mental effects of the current pandemic among medical staff and the general population are critically important, and the pandemic might trigger stress, anxiety, and depression........
Mojtaba Dehghani Firouzabadi, Mozhgan Karbalaie, Atiyeh Javaheri, Maryam Eftekhar, Hossein Fallahzade, Mohammad Javad Mahdinejad Yazdi,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Isthmocele is a uterine scar defect or an opening in the place of a previous cesarean incision that leads to myometer discontinuity and can be diagnosed by hysteroscopy as its gold standard. The probability of forming isthmocele is 61% after one cesarean and 100% after three cesarean. Its common symptoms are abnormal uterine bleeding, pelvic pain, dysmenorrhea, infertility, and dyspareunia. In addition to medical treatment, surgery via laparoscopy or hysteroscopy is a safe and effective treatment for this complication. This study aimed to investigate and compare the results of laparoscopic and hysteroscopic isthmocele repair surgeries in women referred to Shahid Sadoughi Hospital in Yazd due to abnormal bleeding in menstrual intervals as a result of isthmocele.
Methods and Materials: In this pre- and post-experimental study, we included 20 women referred to Shahid Sadoughi Hospital. They entered the study via the census method. The inclusion criteria were age between 18 and 45, abnormal bleeding in menstrual intervals, and having isthmocele. The exclusion criteria entailed irregular menstruation, having an IUD, having coagulation disorder, and having any pathology in the uterine like myoma. The patients underwent laparoscopic or hysteroscopic repair surgeries, and the data were recorded in a questionnaire. Thirty months later, we investigated and compared the patients in terms of uterine myometrium thickness, the volume of menstrual interval bleeding, dysmenorrhea, dyspareunia, and pelvic pain. Ultimately, our data were analyzed with SPSS version 23, employing t-, Chi-square, and Wilcoxon tests.
Results: Within 30 months, 20 patients (11 in the laparoscopy and 9 in the hysteroscopy group) were studied. In laparoscopic surgery, a significant increase was identified in uterine thickness (p = 0.001) and a decrease in menstrual interval bleeding (p = 0.007). Also, improvement in pelvic pain (p = 0.034), dysmenorrhea (p = 0.038), and dyspareunia (p = 0.031) were observed to be significant. In hysteroscopic surgery, decreased menstrual intervals bleeding (p = 0.011) and relief in pelvic pain (p = 0.04) were significantly achieved, but uterine thickness was not considerably different before and after the surgery (p = 0.096). Also, relief in dysmenorrhea (p = 0.999) and dyspareunia (p = 0.157) were not significant.
Conclusion and Discussion: This study shows that repairing isthmocele via hysteroscopy or laparoscopy improves menstrual interval bleeding and pelvic pain. Based on the results of two surgical methods, laparoscopic surgery is a superior choice compared to hysteroscopic surgery.
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