Showing 10 results for Jalili
Ali Mirjalili, Mohammad H Alimohammadian, Rasoul Madani,
Volume 7, Issue 4 (10-2003)
Abstract
The onset of infections by Leishmania parasites mainly caused by amastigote growth inside the macrophages. One of the important properties of lesion-derived amastigote is thought to be the attachment of various host proteins including immunoglobulins on the surface of amastigote. In this study, the presence of immunoglobulins on the surface of lesion-derived amastigotes was detected by Western blotting using three different peroxidase conjugated anti-heavy chain antibodies and peroxidase conjugated anti-mouse IgG antibody. Then, a new technique was developed for isolation of lesion-derived amastigotes. This technique simply consists of a microbiological plate covered by rabbit anti-mouse immunoglobulins. Overnight incubation of the infected cell suspension isolated from mice lesion on such plate at 4°C, was followed by gentle washing and isolation of the amastigotes. The results showed that the surface of amastigote has covered with different amount of immunoglobulins such as IgG, IgM, and IgA detected by pixel analysis software. With this technique, which was comparable with other techniques, pure amastigote was isolated. This is a simple and reliable method for isolation of real amastigotes from lesion of the infected BALB/c mice
Mehrdad Roghani, Mohammad Taghi Joghataie, Mohammad Reza Jalili, Tourandokht Baluchnejadmojarad,
Volume 10, Issue 2 (4-2006)
Abstract
Diabetes mellitus is accompanied with disturbances in learning, memory, and cognitive skills in the human society and experimental animals. Therefore, this research study was conducted to evaluate time-dependent changes in passive avoidance and Y-maze performance in male diabetic rats. For this purpose, male Wistar rats were randomly divided into control and diabetic groups. For induction of diabetes, streptozotocin (STZ) was injected i.p. at a single dose of 60 mg/kg. For evaluation of learning and memory, initial latency (IL) and step-through latency (STL) were determined at the end of 1 st , 2 nd , and 3 rd months using passive avoidance and Y-maze tasks. It was found out that mean IL exhibits a significant increase only at the end of 2 nd (p<0.05) and 3 rd (p<0.01) months. In addition, STL significantly reduced at the end of 2 nd (p<0.05) and 3 rd months (p<0.01). Regarding Y-maze task, alternation score of the diabetic rats was lower than that of the control ones at the end of 1 st (p<0.05), 2 nd (p<0.01), and 3 rd (p<0.01) months as compared to time-matched control group. To conclude, at least one month is strictly required for development of behavioral disturbances in passive avoidance and Y-maze tasks in STZ-diabetic rats.
Fatemeh Amiri, Sedigheh Molaei, Marzie Bahadori, Fatemeh Nasiri, Mohammad Reza Deyhim, Mohammad Ali Jalili, Mohammad Reza Nourani, Mehryar Habibi Roudkenar,
Volume 20, Issue 3 (7-2016)
Abstract
Background: Mesenchymal stem cells (MSCs) have been recently received increasing attention for cell-based therapy, especially in regenerative medicine. However, the low survival rate of these cells restricts their therapeutic applications. It is hypothesized that autophagy might play an important role in cellular homeostasis and survival. This study aims to investigate the regenerative potentials of autophagy-modulated MSCs for the treatment of acute liver failure (ALF) in mice. Methods: ALF was induced in mice by intraperitoneal injection of 1.5 ml/kg carbon tetrachloride. Mice were intravenously infused with MSCs, which were suppressed in their autophagy pathway. Blood and liver samples were collected at different intervals (24, 48 and 72 h) after the transplantation of MSCs. Both the liver enzymes and tissue necrosis levels were evaluated using biochemical and histopathological assessments. The survival rate of the transplanted mice was also recorded during one week. Results: Biochemical and pathological results indicated that 1.5 ml/kg carbon tetrachloride induces ALF in mice. A significant reduction of liver enzymes and necrosis score were observed in autophagy-modulated MSC-transplanted mice compared to sham (with no cell therapy) after 24 h. After 72 h, liver enzymes reached their normal levels in mice transplanted with autophagy-suppressed MSCs. Interestingly, normal histology without necrosis was also observed. Conclusion: Autophagy suppression in MSCs ameliorates their liver regeneration potentials due to paracrine effects and might be suggested as a new strategy for the improvement of cell therapy in ALF.
Ladan Jalalie, Mohammad Jafar Rezaie, Ali Jalili, Mohammad Ali Rezaee, Zakaria Vahabzadeh, Mohammad Reza Rahmani, Mojtaba Karimipoor, Mohammad Saeed Hakhamaneshi,
Volume 23, Issue 3 (5-2019)
Abstract
Background: Mesenchymal stem cells (MSCs) can be used to treat premature ovarian failure (POF). Different methods have already been applied to detect MSCs in tissues. This study aimed to investigate the quantitative distribution of CM-DiI-labeled human umbilical cord vein MSCs (hUCV-MSCs) in different regions of the ovarian tissue of the cyclophosphamide ( CTX )-induced POF in mice. Methods: Adult female C57BL/6 mice (n = 40) were divided into four groups: (1) Mice receiving PBS as control (Ctrl) group; (2) mice receiving hUCV-MSCs intravenously as Ctrl + hUCV-MSCs group; (3) mice receiving CTX intraperitoneally (i.p.) as CTX group; (4) mice receiving CM-DiI-labeled hUCV-MSCs after CTX injection as CTX + hUCV-MSCs group. Histological changes and CM-DiI-labeled hUCV-MSCs distribution were analyzed in the ovarian tissues. Quantitative real-time PCR was performed to detect human mitochondrial cytochrome b (MTCYB) gene in the ovarian tissues of the mice. Results: The mean number of the fluorescent hUCV-MSCs was 20 ± 2.5 (57.1%) in the medulla, 11.3 ± 2.8 (32.2%) in the cortex, and 5.5 ± 1 (15%) in the germinal epithelium of the ovarian tissue (p < 0.05). Moreover, MTCYB gene was detected in the mice ovaries of the CTX + hUCV-MSCs group, but not in other groups. Conclusion: Our findings suggest that the distribution of the transplanted hUCV-MSCs in different regions of the ovarian tissue is not equal, and it is greater in the medulla than the cortex and germinal epithelium. This is the first report of quantitative distribution of MSCs in different regions of ovarian tissue in the POF model.
Manizheh Narimani, Mohammadreza Sharifi, Mohammad Saeed Hakhamaneshi, Daem Roshani, Mohammad Kazemi, Seyed Hossein Hejazi, Ali Jalili,
Volume 23, Issue 6 (11-2019)
Abstract
Background: Acute myelocytic leukemia (AML) is a clonal malignancy resulting from the accumulation of genetic abnormalities in the cells. Human baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5), encodes survivin, is one of only a handful of genes that is differentially over-expressed in numerous malignant diseases including AML. Methods: The BIRC5 was silenced permanently in two AML cell lines, HL‑60 and KG-1, via the CRISPR/Cas9n system. After transfection of CRISPR constructs, genomic DNA was extracted and amplified to assess mutation detection. To evaluate BIRC5 gene expression, quantitative real-time PCR was performed. Also, MTT cell viability and Annexin‑V/propidium iodide flowcytometric staining were performed, and the data were analyzed using the Kolmogorov-Smirnov, Levene's, and ANOVA tests. Results: The results indicated that Cas9n and its sgRNAs successfully triggered site-specific cleavage and mutation in the BIRC5 gene locus. Moreover, suppression of BIRC5 resulted in the reduction of cell viability, and induction of apoptosis and necrosis in HL60 and KG1 suggested that the permanent suppression of BIRC5 remarkably dropped the gene expression and cells viability. Conclusion: This study reinforces the idea that BIRC5 disruption via Cas9n:sgRNAs has favorable effects
on the AML clinical outcome. It thereby can be a promising candidate in a variety of leukemia treatments.
Cyrus Jalili, Ali Ghanbari , Shiva Roshankhah, Mohammad Reza Salahshoor,
Volume 24, Issue 1 (1-2020)
Abstract
Background: The application of methotrexate (MTX) as a chemotherapy agent and immune system suppressant has various side effects. Crocin, a xanthine derivative plant, has many therapeutic benefits. This study was planned to assess the effect of crocin on renal toxicity of MTX in a rat model. Methods: Forty eight rats were divided randomly into eight groups (n = 6), which received normal saline, MTX, crocin, and MTX + crocin for 28 days intraperitoneally. The levels of oxidative stress in kidney and blood serum were measured, and the kidney was analyzed histologically. Results: MTX caused an enhancement in the levels of thiobarbituric acid reactive substances and biochemical marker (creatinine and BUN). Besides, a significant decrease was observed in tissue parameters and antioxidant capacity compared to the normal control group (p < 0.001). The crocin and crocin + MTX decreased the biochemical markers, the levels of thiobarbituric acid reactive species, and tissue parameters considerably at entire dose (12.5, 25, and 50 mg/kg) and enhanced the antioxidant capacity levels compared to the MTX group (p < 0.001). Conclusion: Administration of crocin improves the damage caused by MTX in rats. The crocin by the establishment of balance in the levels of antioxidant prevents the damage to the renal cell membrane, and subsequently the renal damage repairs.
Elmira Firouzi, Vahideh Aghamohammadi, Amirhosein Jalili,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Artificial intelligence (AI) is expected to provide diet-planning solutions via automatic and practical application of professional knowledge, addressing the complexity of optimal diet design. This study aimed to perform a systematic review to evaluate the effect of AI technology in dietary management.
Search Strategy: This study was conducted according to the PRISMA protocol. A comprehensive online search was performed in PubMed-Medline, Scopus, Web of Science, Embase databases, Google Scholar, and the World Health Organization databases from 2014 to September 2024. After searching all databases, 3,893 articles were identified at this stage. Subsequently, after removing 1,078 duplicate articles, 2,815 remained for primary screening. Then after reviewing the titles and abstracts, 34 articles remained to be reviewed for their full text. In the end, in terms of the inclusion criteria, 11 articles were included in this systematic review. The following keywords were used in the search pattern: "Artificial intelligence" OR "Machine learning" AND "Nutrition" OR "Diet" OR "Body weight". We included all English clinical trials and semi-experimental studies to explore the effect of AI technology on dietary management. Exclusion criteria were as follows: (1) in vitro and animal studies, (2) review articles, (3) conference abstracts or other studies lacking full texts, duplicates, letters, and case reports, (4) ongoing studies, and (5) studies whose full text was not available in English.
Results: In the prese review, we investigated 11 clinical trial and semi-experimental studies involving 5,366 participants across intervention and control groups. Two studies were conducted on healthy individuals, one study on obese children, five studies on obese adults, two studies on patients with diabetes, one study on patients with irritable bowel syndrome, and one study on patients receiving bariatric care. The findings from all studies indicated that AI, dietitians, and nutrition researchers were associated with improved weight management.
Conclusion and Discussion: The effect of AI on weight loss and health improvement is undeniable, and numerous studies have proven its effects. AI can facilitate the work of dietitians and nutrition researchers in dietary treatment for obesity and diabetes.
Fatemeh Mirjalili, Shar Rafiee,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Metabolic syndrome (MetS) is a cluster of conditions that elevate the risk of atherosclerotic cardiovascular disease. The metabolic disorders that characterize MetS include central obesity, hypertension, high triglyceride levels, decreased levels of high-density lipoprotein cholesterol (HDL-C), and increased fasting blood glucose levels. Due to the sedentary lifestyle and consumption of unhealthy diets, the prevalence of MetS has significantly increased. In this regard, a healthy diet based on dietary approaches to stop Hypertension (DASH) can be an appropriate intervention that can improve metabolic problems. Available evidence supports the hypothesis that adherence to the DASH diet may be associated with the Mets; however, the results of previous studies have not been entirely consistent. We conducted a systematic review to assess the relationship between DASH diet and MetS adherence.
Search Strategy: A systematic search was conducted in online databases, including PubMed, Web of Science, Scopus, and Google Scholar, up to June 2024 without publication date or language restrictions. Search terms included "DASH" OR "dietary approaches to stop hypertension" AND "MetS" OR "Metabolic syndrome." Then, appropriate prospective cohort studies were selected based on the inclusion and exclusion criteria.
Results: After screening related articles, considering the inclusion and exclusion criteria, seven prospective cohort studies with a total sample size of 314,196 people and 14,752 cases of MetS were included in the systematic review. The cohort studies showed an inverse association between adherence to the DASH diet and the risk of MetS. In addition, the results of a cohort study on patients with diabetes, dyslipidemia, and hypertension showed that adherence to the DASH diet reduces the risk of Mets in this population.
Conclusion and Discussion: Our findings demonstrate that adherence to the DASH diet can be associated with a lower risk of MetS and its components. However, further studies with a randomized controlled trial design are needed.
Amir Mohammad Nejadsheikh, Amir Hosein Jalili, Maryam Mamlekatkhah,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: As the first line of defense against diseases, nurses perform exhausting services, which in the long run causes nurses to burn out. In order to reduce the pressure on jobs, facilities such as artificial intelligence (AI) can be used. The purpose of this research was to determine the impact of AI in improving nursing services.
Search Strategy: In the current review, articles published from 2000 to October 2023 were used in the databases PubMEd, SID, Science Direct, Magiran, Google scholar. The keywords used were [("Artificial Intelligence" OR "Machine Intelligence") AND ("Nursing services" OR "Nursing")] and ("Nursing" "Artificial Intelligence" "Services"). The criteria for including articles in the review were that the clinical trial must be a controlled case, available in both Farsi and English, and must conforms to the established criteria.
Results: Based on the searches conducted in the specified databases, 339 articles were identified. Among these, the following topics were included in the study and reviewed: (1) the promotion of management in critical situations, and (2) the Promotion of nursing.
Conclusion and Discussion: According to the available studies, AI has enhanced the work of nurses by reducing errors, increasing focus, and improving patient satisfaction with the services provided. Some studies indicates that AI contributes to the overall health of both nurses and patients while also decreasing the time required for nursing tasks. However, certain studies have noted that AL is unable to perform complex and specialized nursing procedures, such as resuscitation. Additionally, many studies emphasize that nursing ethics are often overlooked in the application of AL due to its rapid advancement. The lack of sufficient research and the critical need to prevent burnout among nurses suggest that further studies in this area are necessary.
Tooba Farhadi, Ali Jalili, Fatemeh Hajizadeh Sharafabad,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Nonalcoholic fatty liver disease (NAFLD) may lead to a progressive liver disease with the risks for fibrosis and carcinoma. As a natural antioxidant, Alpha-lipoic Acid (ALA) may increase total antioxidant capacity and mitochondrial biogenesis, influencing liver function and possibly NAFLD. This study aimed to systematically review the effects and potential mechanistic pathways of ALA in NAFLD.
Search Strategy: This systematic review was carried out based on the guidelines of the 2010 PRISMA statement. Electronic databases, including PubMed, Web of Science, and Scopus, were searched until June 2024. Original studies published in English-language journals that investigated the effects of ALA on NAFLD were considered. Key search terms were thioctic Acid, alpha-lipoic acid, lipoic Acid, and α-lipoic Acid in combination with liver function tests, liver fat, hepatic steatosis, liver steatosis, liver steatoses, NASH, NAFLD, steatohepatitis, nonalcoholic fatty liver disease, and fatty liver. Two reviewers independently extracted data on study characteristics, methods, and outcomes.
Results: A total of 1297 articles were found in the first phase of the search; from these, 30 met the inclusion criteria: 3 in vitro, 21 animal, and six human studies. All three in vitro studies demonstrated the lowering effects of ALA on fat accumulation in the liver. 18 of 21 animal studies indicated a significant decrease in hepatic steatosis mainly by affecting the hepatic genes such as FOXO1, SREBP1, PPARs α or γ, and FASN, which are involved in the lipolysis and β-oxidation of lipids. However, two animal studies showed a significant increase in hepatic lipids and serum levels of alanine transaminase (ALT) and aspartate transaminase (AST). Half of the clinical trials also detected a significant decrease in hepatic steatosis.
Conclusion and Discussion: The studies showed that ALA might improve body weight, insulin resistance, inflammatory status, oxidative stress, and hepatocellular lipolysis and subsequently relieve NAFLD. Although the experimental evidence supports the positive effects of ALA on hepatic steatosis, further clinical trials focusing on the effects of ALA on fatty liver are warranted.
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