Showing 4 results for Enayati
Ahmad Ali Enayati, Farzad Motevalli Haghi,
Volume 11, Issue 4 (10-2007)
Abstract
The German cockroach is an important household insect pest mechanically involved in transmission of a variety of diseases to humans. Different classes of insecticides have extensively been used for its control leading to insecticide resistance development. Hence, for an optimal control of this pest, the status and underlying mechanisms of insecticide resistance should be studied in this group of insects. Methods: Adult German cockroaches were collected from Imam and Bouali Cina Hospitals (Sari, Iran) and subjected to bioassay using jar test method. The results were compared to those of a susceptible laboratory strain. Biochemical assays of esterases, monooxigenases and glutathione S-transferase (GST) levels were undertaken on German cockroaches from Imam and Bouali Cina Hospitals and the results were compared deltamethrin 19.64 ± 2.9, 18.66 ± 3.45 and 8.64 ± 0.62 min for cypermethrin, respectively. The mean to a susceptible laboratory strain. Results: The LT50 values of the three strains were 20.24 ± 2.2, 19.87 ± 2.3 and 8.89 ± 0.26 for permethrin 19.3 ± 3.05, 17.6 ± 0.68 and 8.8 ± 0.99 for a-esterase activity of Imam and Bouali Cina Hospitals and susceptible strains were 6.941 × 10-4, 6.940 × 10-4 and 8.01 × 10-5 nmol/min/mg protein the mean b-esterase activity in those strains were 5.8 × 10-4, 4.25 × 10-4 and 7.28 × 10-5 nmol/min/mg protein the mean content of p450 in the above-mentioned strains were 5.64 × 10-6, 1.89 × 10-6 and 1.2 × 10-6 nmol/mg protein the mean GST activity were 6.66 × 10-2, 0.102 and 5.72 × 10-2 mmol/min/mg protein, respectively. Conclusion: The LT50 values and also the mean activity of all enzyme groups in field strains were significantly different from those of the susceptible strain, indicating a vigour tolerance to insecticides and pyrethroids in particular. Hence, insecticide resistance monitoring techniques should be put in place and also resistance management strategies and measures should be considered implementing in the area.
Solmaz Agha Amiri, Najmeh Zarei, Somayeh Enayati, Mohammad Azizi, Vahid Khalaj, Soraya Shahhosseini,
Volume 22, Issue 1 (1-2018)
Abstract
Background: Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. Methods: Four variables (cell optical density at induction, IPTG concentration, induction temperature, and induction time) were tested using experimental design. Results: Our findings demonstrated that among the examined variables, only the induction time had a significant positive effect on the protein expression yield. Conclusion: Experimental design was successfully applied in this study. The optimized condition obtained in the current study can be applied in future commercial production of this novel fusion protein.
Najmeh Zarei, Hosnieh Ghasemi, Mahsa Nayebhashemi, Mozhgan Zahmatkesh, Monire Jamalkhah, Nafiseh Moeinian, Zahra Mohammadi, Somayeh Enayati, Vahid Khalaj,
Volume 25, Issue 4 (7-2021)
Abstract
Background: The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant proteins, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Loss of supression (Los1) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which its deletion extends replicative lifespan. Herein, a los1-deficient strain of P. pastoris was generated and characterized. Methods: A gene disruption cassette was prepared and transformed into an anti-CD22-expressing strain of P. pastoris. A δ los1 mutant was isolated and confirmed. The drug sensitivity of the mutant was also assessed. The growth pattern and the level of anti-CD22 single-chain variable fragment (scFv) expression were compared between the parent and mutant strains. Results: The los1 homologue was found to be a non-essential gene in P. pastoris. Furthermore, the susceptibility of los1 deletion strain to protein synthesis inhibitors was altered. This strain showed an approximately 1.85-fold increase in the extracellular level of anti-CD22 scFv (p < 0.05). The maximum concentrations of total proteins secreted by δ los1 and parent strains were 125 mg/L and 68 mg/L, respectively. Conclusion: The presented data suggest that the targeted disruption of los1 homologue in P. pastoris can result in a higher expression level of our target protein. Findings of this study may improve the current strategies used in optimizing the productivity of recombinant P. pastoris strains.
Aliasghar Asgarani, Melika Behvand, Pardis Enayati, Sanaz Bastan, Omid Sadeghi,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Kidney stones are a common urological disease that causes significant pain. The most prevalent kind of kidney stone is calcium oxalate, which is formed in Randall's plaque on the papillary surfaces of the kidney. Stone formation involves supersaturation, crystal nucleation, growth or aggregation, and their retention in the kidney or renal collecting system, leading to additional aggregation. The effect of dietary factors on this disease has been the subject of various studies. We aimed to perform a systematic review of observational studies on the associations of coffee and tea consumption with the risk of kidney stones in adults.
Search Strategy: PubMed, Scopus, and Web of Science online databases were systematically searched using "Tea," coffee," and "kidney stone". The Cochrane risk of bias tool was used to perform quality assessment.
Results: In the current systematic review, we found 12 observational studies, five of which assessed coffee consumption for kidney stones. Among them, two studies revealed a significant inverse association between coffee consumption and the risk of kidney stones. Such protective association was also seen in 6 studies on tea consumption and the risk of kidney stones. The protective effect of these two drinks can be explained by several mechanisms, such as increased water intake, which dilutes the mineral concentration in the urine. Other possible mechanisms are related to the presence of caffeine with diuretic effects, leading to increased urine flow rate and calcium excretion. Furthermore, it can reduce the adhesion of calcium oxalate crystals to renal tubular cells and translocate annexin A1 (a crystal-binding protein) from the apical surface of renal tubular cells into the cytoplasm. Thus, the ability of the cells to bind crystals is subsequently reduced. The inhibitory effects of caffeine may be enhanced by other compounds present in coffee and tea, such as antioxidants. The oxidant-antioxidant imbalance damages oxidative stress and is essential in kidney stone development.
Conclusion and Discussion: This review study supports a protective role for coffee and tea, mainly green tea with lower oxalate content and higher antioxidants, against stone formation.
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