Showing 7 results for Azami
Siamak M Samiee, Nasrin Moazami, Saeid Haghighi, Farzaneh Aziz Mohseni, Saeid Mirdamadi, Mohammad Reza Bakhtiari,
Volume 7, Issue 1 (1-2003)
Abstract
In the present study, 110 fungal strains of Persian Type Culture Collection (PTCC) including some selected strains isolated in various screening projects were tested for their potentiality to produce lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reduc-tase), the rate-limiting enzyme of cholesterol biosynthesis. The fungal strains were cultivated in a two-stage submerged fermentation followed by screening by TLC. All positive results were evaluated by confirmatory HPLC. Nine species of four genera were found to be lovastatin producers. Aspergillus terreus was the best lovastatin producing strain with a level of 55 mg lovastatin per liter of screening production medium
Mohammad Faezi Ghasemi, Mohammad Reza Bakhtiari, Masoud Fallahpour, Ashrafossadat Noohi, Nasrin Moazami, Zohreh Amidi,
Volume 8, Issue 1 (1-2004)
Abstract
In this study, urease production was investigated among thirteen strains of Aspergillus niger seven strains isolated from soils of Semnan province in Iran and six strains obtained from Persian Type Culture Collection (PTCC). The enzyme production was screened in two submerged media quantitatively. The registered PTCC 5011 and the native S31 strains showed more urease production than the other eleven strains. The maximum enzyme productions by PTCC 5011 and S31 strains were 106 and 109 U.g-1dry mass in submerged culture, respectively. Also, we used two solid media for screening all of the strains for urease production semi quantitatively. Due to the acceptable correlations between the two methods, the latter can be used as an ancillary method to mass screening of urease production by filamentous fungi.
Saeed-Reza Pakzad, Soheila Ajdary, Nasrin Moazami, Saeed Haghighi,
Volume 9, Issue 2 (4-2005)
Abstract
b-cyclodextrin glucosyl transferase (b-CGTase) hydrolyses starch to produce b-cyclodextrin by transglycosylation (cyclization) activity. The conventional method for detection of b-CGTase activity is based on detecting starch hydrolysis by iodine staining. This method reveals all amylolytic enzymes, but can not discriminate them. In the present study, we introduce a new method for specific detection of b-CGTase activity and its specific product i.e. b-cyclodextrin by polyacrylamide gel. In this method, solution containing b-CGTase is subjected to electrophoresis on 10% polyacrylamide gel. Then, the gel is covered with an indicator gel containing phenolphthalein, soluble starch, and agar. After a short incubation, sodium carbonate solution is added and the positive result is indicated by the emergence of a colorless band in the red context of the indicator gel. Since the production of b-cyclodextrin via cyclization is unique to b-CGTase, the emergence of clear bands is indicative of b-CGTase presence. However, in conventional starch-iodine method, all amylolytic enzymes including b-CGTase give positive results. Therefore, for detection of b-CGTase, the phenolphthalein indicator gel method, compared to the starch-iodine method, is more specific
Abbasali Karimpour Malekshah, Mahmoud Heidari, Kazem Parivar, Nasrin Sadat Azami,
Volume 18, Issue 1 (1-2014)
Abstract
Background: This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. Methods: The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base medium as control group (n = 113), (ii) base medium supplemented with 30 ng/ml Activin A (n = 115), (iii) base medium co-cultured with mouse embryonic fibroblast (n = 113), and (iv) base medium supplemented with 30 ng/ml Activin A and co-cultured with fibroblast (n = 115). Rate of growth, survivability, antrum formation, ovulation, embryonic development and steroid production were evaluated. Analysis of Variance and Duncan test were applied for analyzing. Results: Both co-culture and co-culture + Activin A groups showed significant difference (P<0.05) in growth (on days 4, 6, and 8 of culture period) and survival rates. However, there was no significant difference in antrum formation, ovulation rate, and embryonic development of ovulated oocytes. There were significant differences (P<0.05) in the estradiol production on days 8, 10, and 12 between co-culture + Activin A and the control group. Progesterone production also was significant (P<0.05) in co-culture + Activin A group on days 6, 8, 10, and 12 compared to control group. Conclusion: Fibroblast co-culture and Activin A promoted growth and survivability of preantral follicles. However, simultaneous use of them was more efficient.
Meghdad Abdollahpour-Alitappeh, Majid Lotfinia, Sepand Razavi-Vakhshourpour, Saeed Jahandideh, Hamid Najminejad, Koushan Sineh Sepehr, Reza Moazami, Elnaz Shams, Mahdi Habibi-Anbouhi, Mohsen Abolhassani,
Volume 21, Issue 4 (7-2017)
Abstract
Background: Reduction/alkylation is one of the leading strategies for the development of antibody drug conjugates (ADCs). Precise control of the reduction process would not only yield a defined number of free thiols per antibody but also result in development of more homogenous conjugates. Methods: In the present study, we investigated the effect of various dithiothreitol (DTT) concentrations, temperature conditions, and DTT exposure times on antibody reduction. After antibody reduction, the Ellman's test and SDS-PAGE analysis were used to evaluate free thiols produced and confirm the reduction process, respectively. Results: DTT concentration seems to be a potential factor in the reduction process. Concentrations of 0.1, 1, 5, 10, 20, 50, and 100 mM DTT at 37°C for 30 minutes resulted in approximately 0.4, 1.2, 5.4, 7, 8, 8, and 8 thiols per antibody, respectively. Conclusion: Optimized site-specific conjugation can provide better process control and reproducibility for the development of disulfide-based ADCs.
Amirhossein Khalildokht, Farzaneh Monazami, Borhan Rahimirad, Sevana Daneghian, Mohammad Heidari,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: It is essential to explore potential therapies due to the significant impact of migraine-related disabilities. The role of metals in this context warrants attention because of their importance in clinical medicine for both diagnosis and treatment. Zinc, is particular, stands out as a vital element and is increasingly recognized for its role in maintaining health. This study aimed to conduct a systematic review and meta-analysis to thoroughly evaluate and compile international data on the relationship between zinc intake and migraine headaches.
Search Strategy: Until May 8, 2024, we thoroughly searched the PubMed, Scopus, MEDLINE, and EMBASE databases using relevant search phrases associated with "Migraine" and "Zinc". There were no limitations on the language or the publication date. Review Manager 5.4 was used to conduct statistical analyses to evaluate the impact of zinc supplementation on the frequency and duration of migraine attacks.
Results: We examined data from a comprehensive study involving five trials. It was found that the combined effect of zinc on reducing the frequency of migraine attacks was -2.03 (95% CI: -3.61 and -0.46), showing a significant decrease. Moreover, there was a trend toward reduction, as shown by the pooled impact of zinc, which was -1.37 (95% CI: -3.03 and 0.28), in reducing the duration of these attacks.
Conclusion and Discussion: Our findings demonstrate that taking zinc supplements significantly reduces the frequency of migraine attacks. However, the evidence regarding its effect on the duration of the attacks is less compelling. Consequently, these results suggest that zinc supplementation may serve as a potential intervention for mitigating the frequency of migraine episodes. Further investigation and clinical trials to validate its efficacy and determine optimal dosage regimens.
Maziar Afshar, Yousef Moradi, Maryam Afraie, Farhad Moradpour, Kamran Zamani, Mobin Azami,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Metabolic syndrome (MetS) is a prevalent endocrine disorder characterized by a cluster of metabolic abnormalities, including abdominal obesity, dyslipidemia, hypertension, and impaired glucose metabolism. Early diagnosis and screening of this disorder are crucial for its effective management. The triglyceride-glucose (TyG) index, derived from fasting triglycerides and glucose levels, has emerged as a potential alternative marker for insulin resistance and MetS.
Methods and Materials: This cross-sectional study utilized data from the Dehgolan Prospective Cohort Study, which involved 3,800 participants aged 35 to 70 from Western Iran. MetS was defined using WHO criteria. The TyG index and its variants and anthropometric indices were calculated and evaluated for their diagnostic performance in identifying MetS using regression analysis, receiver operating characteristic curves, and area under the curve (AUC).
Results: The prevalence of MetS in the study population was 35.19%. In the total population, the TyG-WHtR (waist-to-height ratio) index exhibited the highest AUC of 0.86 (sensitivity: 84.56%, specificity: 72.23%). In the male population, the TyG-WC (waist circumference) index had the highest AUC of 0.90 (sensitivity: 79.29%, specificity: 85.4%), while in females, the TyG index alone ranked first with an AUC of 0.87 (sensitivity: 76.12%, specificity: 87.1%).
Conclusion and Discussion: The TyG index and its anthropometric variants demonstrated high diagnostic accuracy for MetS, with the TyG-WHtR and TyG-WC indices exhibiting superior performance in the total and male populations, respectively. The TyG index alone exhibited the highest diagnostic value in females. These findings suggest the potential utility of the cost-effective TyG index, alone or combined with anthropometric parameters, as a screening tool for early MetS detection, particularly in resource-limited settings. However, further validation across diverse populations is warranted.
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