Showing 3 results for Attari
Youcef Remram, Mokhtar Attari, Noureddine Ababou,
Volume 11, Issue 3 (7-2007)
Abstract
Accurate measurements of physical characteristics of bone are essential for diagnosis, assessment of change following treatment, and therefore, indirectly, for evaluation of new forms of therapy. This is particularly true of osteoporosis and aging skeleton, in which fractures occur easily. Methods: In this study an ultrasonic system was set-up and calibrated on Plexiglas tubes of variable thickness then used to detect the cortical bone thickness change in calf and bovine adult femurs. Lamb waves have been generated and detected using a pair of Piezoelectric point transducers (transmitter and receiver) operating at 60 kHz in contact with the surface of the bone. Results: A link has been established between the ultrasound velocity and the bone thickness. On the other hand, the density variation has been also investigated by the simulation of the bone decalcification chemically. The results show that the velocity is very sensitive to both thickness and density, its value reduces as the cortical bone thickness and density decrease. Conclusion: This technique might be considered in the attendance of certain bone diseases expressing itself by gradual change in physical properties.
Farnoosh Attari, Houri Sepehri, Ladan Delphi, Bahram Goliaei,
Volume 13, Issue 4 (10-2009)
Abstract
Background: Pectin is composed of complex polysaccharides that can inhibit cancer metastasis and proliferation with no evidence of toxicity. In the present study, the apoptotic and necrotic effects of pectic acid (PA) on the rat pituitary GH3/B6 tumor cells has been investigated. Methods: GH3/B6 cells were cultured in the Ham’s F12 medium enriched with 15% horse serum and 2.5% fetal bovine serum for 3 days. Then, they were treated by various amounts of PA in different periods (6, 24 and 48 hours). Bromocriptine was used as positive control and the cell viability was detected by MTT test. The nuclear morphology of cells was explored by florescent stains including acridine orange (AO)/ethidium bromide (EB). In addition, percentages of apoptotic and necrotic cells were studied with triphosphate nick-end labeling (TUNEL) assay, cell cycle analysis and propidium iodide (PI) staining. Results: Long-term incubation with PA results in increased cell death and DNA damage as detected by MTT assay and AO/EB staining. TUNEL assay showed that PA (100 µg/ml to 1 mg/ml) could induce apoptosis in a dose-dependent manner, while higher concentrations of PA (2.5 and 5 mg/ml) induced necrosis which was confirmed by PI staining. Furthermore, cell cycle analysis indicated that PA induced sub G1 events, and DNA fragmentation was also correlated with the number of the apoptotic cells. Conclusion: It can be concluded that PA is responsible for apoptosis in the rat pituitary tumor cells. Therefore, one may suggest that this group of polysaccharides can be used in treatment of pituitary tumors.
Arman Habibi, Negin Letafatkar, Soheil Hassanipour, Fariborz Mansour Ghanaei, Farahnaz Joukar, Nazila Sattari, Sara Nobakht, Ehsan Amini Salehi, Mohammad Hossein Keivanlou,
Volume 28, Issue 0 (Supplementary 2024)
Abstract
Introduction: Type 2 diabetes mellitus (T2DM) poses a significant global health challenge due to various lifestyle factors contributing to its prevalence and associated complications. Chronic low-grade inflammation, characterized by elevated levels of inflammatory markers such as C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), plays a pivotal role in the pathogenesis of T2DM. Modulation of the gut microbiota through microbiome-targeted therapy (MTT), including probiotics, prebiotics, and synbiotics, has emerged as a potential strategy to mitigate inflammation and improve metabolic outcomes in T2DM.
Methods and Materials: A systematic review and meta-analysis were conducted following PRISMA guidelines to evaluate the impact of MTT on inflammatory markers in patients with T2DM. Searches were performed in PubMed, Scopus, and Web of Science databases up to December 2023, with inclusion criteria limited to English-language meta-analyses of randomized controlled trials (RCTs) assessing the effects of probiotics, prebiotics or synbiotics on inflammatory markers in T2DM patients.
Results: Ten meta-analyses met the inclusion criteria, comprising studies investigating the effects of various MTT interventions on CRP, IL-6, and TNF-α levels in T2DM patients. Meta-analysis results indicated significant reductions in CRP (SMD: -0.070; 95% CI: -0.119 to -0.020) and TNF-α (SMD: -0.370; 95% CI: -0.554 to -0.186) levels following MTT, while IL-6 reductions (SMD: -0.070; 95% CI: -0.269 to 0.129) did not reach statistical significance. However, heterogeneity in study quality, intervention protocols, and participant demographics posed challenges in interpretation.
Conclusion and Discussion: Despite observed improvements in inflammatory markers with MTT, limitations, including heterogeneity in study quality and intervention protocols, underscore the need for further research to confirm efficacy and elucidate mechanisms. Future studies addressing these limitations, including diversity in dosage, supplement types, and bacterial strains, are essential for enhancing the reliability and applicability of MTT in T2DM management.
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