Mojtaba Moosavian, Bahram Fathollahzadeh, Kazem Amoli, Nasrin Moazzami,
Volume 2, Issue 2 (3-1998)
Abstract
In this survey, 187 samples collected from 9 different sources of water in Tehran such as: water coolers, neonates incubators, water bathes (37° C), little pools in parks or squares, cooling towers, hot or cold water taps, were examined for Legionnaires' disease agents. Inoculating water samples on selective and nonselective media resulted in isolation of several strains of gram negative bacteria. Among them, seven strains of Legionella pneumophilla which did not grow on any of the above media, were identified by indirect fluorescent antibody technique (IFA) and biochemical tests. Following confirmation by "Center National De Reference Des Legionelloses, Faculte of Medicine Alexis Carrel, Lyon", the Center identified Legionella pneumophila of serogroups of 1, 8 and 10. Out of 6 hospitals, 5 showed contaminated water having pneumonic Legionellosis agents.
Hossein Mostafavi, Narges Amoli, Elham Ghasemloo, Meysam Forouzandeh, Masoumeh Hosseini, Mehdi Eskandari,
Volume 26, Issue 5 (9-2022)
Abstract
Introduction: Brain ischemia often leads to the chloride gradient alternations, which affects volume regulation and neuronal survival. Increase in NKCC1 expression and reduction in KCC2 level under ischemic condition results in inflammation and neuronal death. In this study, we investigated the effect of mimic miRNA and coenzyme Q10 (CoQ10) on the expression of cation-chloride cotransporters (CCCs) (NKCC1 and KCC2) after cerebral ischemia.
Methods: In this study, cerebral ischemia was modeled using the middle cerebral artery occlusion method. Rats were randomly divided into six groups: sham, model, negative control, vehicle, and the first and second treatments. In the Sham group, ischemia was not induced, and no treatment was performed. In the Model group, ischemia induction was performed, and other groups, in addition to ischemia induction, received Scramble miRNA, Ethanol, mimic miRNA-149-5p and CoQ10, respectively. Each group was divided into three subgroups to assess the volume of the tissue damage and neurological deficits scores (NDS) in subgroup 1, brain water content in subgroup 2, level of miRNA-149-5p and CCC expressions in subgroup 3.
Results: Our data suggested that the use of mimic miRNA and Q10 increased the level of miRNA-149 and KCC2 expression and decreased NDS, NKCC1 expression, brain water content, and infract volume.
Conclusion: Findings of this study suggest that the mimic miRNA and Q10 may have neuroprotective effects through reducing infract volume and brain water content and modulating the expression of CCCs after brain ischemia.
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