Volume 2, Issue 1 (1-1998)                   ibj 1998, 2(1): 21-25 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Oloomi M, Bouzari S, Rechinsky V O. PCR-mediated Expression of the Human GM-CSF Gene in Escherichia coli. ibj. 1998; 2 (1) :21-25
URL: http://ibj.pasteur.ac.ir/article-1-888-en.html
Four exons of the human genomic GM-CSF gene were assembled together using gene splicing by overlap extension (SOE) method. The resulting nucleotide sequence was cloned in the pET23a(+) ex‌pression vector under the control of strong bacteriophage T7 transcription and translation signals. The construct obtained was Transferred into the E. coli strain, BL21(DE3) pLysS and IPTG was used for induction of GM-CSF gene and production of the target protein. one mg of protein per liter of cell culture, was obtained as revealed by ELISA.
Type of Study: Full Length |

Add your comments about this article : Your username or Email:

© 2021 All Rights Reserved | Iranian Biomedical Journal

Designed & Developed by : Yektaweb