Iranian
Biomedical Journal
Volume 3, Issue 3 And 4 (7-1999)
IBJ 1999, 3(3 And 4): 99-101 |
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Ayatollahi M, Malek-Hosseini Z, Imani M, Joubeh S, Mortazavi H, Ghaderi A. Development of an ELISA Based Method for the Detection of Pemphigus vulgaris Autoantibodies. IBJ 1999; 3 (3 and 4) :99-101
URL: http://ibj.pasteur.ac.ir/article-1-842-en.html
URL: http://ibj.pasteur.ac.ir/article-1-842-en.html
Maryam Ayatollahi 
, Zahra Malek-Hosseini , Mohammad-Taghi Imani , Sohaila Joubeh , Hossein Mortazavi , Abbas Ghaderi
, Zahra Malek-Hosseini , Mohammad-Taghi Imani , Sohaila Joubeh , Hossein Mortazavi , Abbas Ghaderi
Abstract:
For the evaluation of autoantibodies in Pemphigus vulgaris (PV), an indirect ELISA assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (M.W.) of 130-160 kDa. The evaluation of Pemphigus IgG, IgM and IgA autoantibodies in 75 patients and 50 control sera indicated that this indirect ELISA assay was a useful method for the detection of Pemphigus autoantibodies. Data of this study suggested that the mean OD (Optical Density) of specific IgG autoantibody was elevated in PV patients compared with the controls. The percentages of the total serum IgM and IgA antibodies were not statistically significant between patients and controls.
Type of Study: Full Length/Original Article |
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