:: Volume 16, Issue 3 (7-2012) ::
ibj 2012, 16(3): 139-133 Back to browse issues page
A DNA Vaccine against Escherichia coli O157:H7
Kowsar Shariati Mehr , Seyed Latif Mousavi * , Iraj Rasooli , Jafar Amani , Masoumeh Rajabi
Abstract:  
Background: Infection with Escherichia coli O157:H7 rarely leads to bloody diarrhea and causes hemolytic uremic syndrome with renal failure that can be deadly dangerous. Intimin, translocated Intimin receptor (Tir), and enterohemorrhagic E. coli (EHEC) secreted protein A (EspA) proteins are the virulence factors expressed by locus of enterocyte effacement locus of EHEC. This bacterium needs EspA as a conduit for Tir delivery into the host cell and the surface arrayed Intimin, which docks the bacterium to the translocated Tir. Methods: Here we used triplet synthetic gene (eit) which was designed from three genes: espA coding EspA 120 lacking 36 amino acids from the N-terminal of the protein, eae coding Intimin constructed of 282 amino acids from the C-terminal and tir coding Tir 103, residues 258-361 which interacts with Intimin. The multimeric gene was cloned in two eukaryotic vectors pAAV-multiple cloning site-green fluorescent protein and pCI-neo. The pAAV was used for gene expression assay in cell line 293T and pCI-neo-EIT (EspA, Intimin, Tir) was used as DNA vaccine in mice. Test groups were injected intramuscularly with pCI-neo-EIT four times and mice control group was injected under the same conditions with PBS or pCI-neo vector. Results: The titration of serums showed that BALB/c mice were successfully immunized with DNA vaccine compared to control groups and also they were protected against challenges of live oral using E. coli O157:H7. Conclusion: The results suggest that the DNA vaccine could induce protective immunity either alone or in combination with purified antigens to reduce EHEC infection.
Keywords: DNA vaccines, Escherichia coli O157:H7, Virulence factors
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Type of Study: Full Length | Subject: Related Fields
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Shariati Mehr K, Mousavi S L, Rasooli I, Amani J, Rajabi M. A DNA Vaccine against Escherichia coli O157:H7. ibj. 2012; 16 (3) :139-133
URL: http://ibj.pasteur.ac.ir/article-1-734-en.html


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