:: Volume 6, Issue 1 (1-2002) ::
IBJ 2002, 6(1): 25-29 Back to browse issues page
Effects of Protoplast Fusion on δ-endotoxin Production in Bacillus thuringiensis Spp. (H14)
Shamsi Yari , Davoud Nouri Inanlou , Fatemeh Yari , Maryam Saleh , Behrokh Farahmand , Azim Akbarzadeh
In this study, mutant forms of Bacillus thuringiensis spp. israelensis (H14) were produced. These mutants were identified when the cells were cultured on chloramphenicol plates and stained with crystal violet. Protoplasts of the mutants were isolated by enzymatic digestion (lysozyme) of the cell walls at the presence of an osmotic stabilizer. The protoplasts were induced to fuse to each other in the presence of PEG 6000. The frequency of regeneration and recombination was 80% and 2´10-4, respectively. In order to survey the effect of protoplast fusion on production of toxin, anti-serum against pure toxin was raised in rabbit and was used in single radial immunodiffusion. The comparison of d-endotoxin concentration between B. thuringiensis fusion and the wild type strains showed that B. thuringiensis fusion has 1.48 time more toxin than wild type.
Keywords: Bacillus thuringiensis, Protoplast fusion, ELISA
Full-Text [PDF 84 kb]      
Type of Study: Full Length |
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >

XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yari S, Nouri Inanlou D, Yari F, Saleh M, Farahmand B, Akbarzadeh A. Effects of Protoplast Fusion on δ-endotoxin Production in Bacillus thuringiensis Spp. (H14). IBJ. 2002; 6 (1) :25-29
URL: http://ibj.pasteur.ac.ir/article-1-572-en.html

Volume 6, Issue 1 (1-2002) Back to browse issues page
Persian site map - English site map - Created in 0.17 seconds with 37 queries by YEKTAWEB 3647