:: Volume 8, Issue 3 (7-2004) ::
IBJ 2004, 8(3): 161-165 Back to browse issues page
Modified DNA Extraction for Rapid PCR Detection of Methicillin-Resistant Staphylococci
Aziz Japoni , Abdolvahhab Alborzi , Manoochehr Rasouli , Bahman Pourabbas
Abstract:  

Nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. The aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. The presence or absence of the methicillin-resistance gene in 115 clinical isolates of Staphylococcus aureus and 50 isolates of Coagulase Negative Staphylococci (CNS) was examined by normal PCR. DNA extraction for PCR performance was then modified by omission of achromopeptadiase and proteinase K digestion, phenol/chloroform extraction and ethanol precipitation. All isolates with MIC>8 mg/ml showed positive PCR. No differences in PCR detection have been observed when normal and modified DNA extractions have been performed. Our modified DNA extraction can quickly detect methicillin-resistant staphylococci by PCR. The advantage of rapid DNA extraction extends to both reduction of time and cost of PCR performance. This modified DNA extraction is suitable for different PCR detection, when staphylococci are the subject of DNA analysis

Keywords: Methicillin-resistant staphylococci, PCR, DNA extraction
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Type of Study: Short Communication |
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Japoni A, Alborzi A, Rasouli M, Pourabbas B. Modified DNA Extraction for Rapid PCR Detection of Methicillin-Resistant Staphylococci. IBJ. 2004; 8 (3) :161-165
URL: http://ibj.pasteur.ac.ir/article-1-503-en.html


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