:: Volume 14, Issue 4 (11-2010) ::
IBJ 2010, 14(4): 178-185 Back to browse issues page
The Effect of “Teucrium polium L.” Extracts on Insulin Release from in situ Isolated Perfused Rat Pancreas in a Newly Modified Isolation Method: the Role of Ca2+ and K+ Channels
Sayid Mahdi Mirghazanfari , Mansoor Keshavarz , Fatemeh Nabavizadeh , Nepton Soltani , Mohammad Kamalinejad
Abstract:  
Background: “Teucrium polium L.” (TP) has been long recommended in Iranian folk medicine for its anti-diabetic activities. We attempt here to evaluate the effect of TP extract on insulin secretion in rat pancreas. Methods: Rat pancreas was isolated in situ and perfused with Krebs solution containing low glucose (LG, 2.8 mM) or high glucose (HG, 16.7 mM) as perfusate. The aqueous extract (Aq. E) and methanolic extract (Met. E) of TP aerial parts and two partition fractions of Met. E were added to perfusate to evaluate insulin release. Diazoxide (DZX) and verapamil (VPM) were also used for assessing the probable mechanism of the effects. In each experimental group, the peak and baseline of insulin levels in effluent samples were compared. The GC/MS analysis was carried out to detect active ingredients in the extracts. Results: Adding Met. E to the LG caused a significant increase (P<0.05) in insulin release from the basal level of 0.17 ± 0.05 µg/l to a peak value of 3.94 ± 1.29 µg/l. when Met. E was introduced to the HG, there was a further protracted stimulation of insulin release from 2.15 ± 1.35 µg/l to 6.16 ± 0.52 µg/l. Both DZX and VPM when added separately to the LG, led to inhibition of Met. E induced insulin secretion. The Aq. E and fractions had no significant effect on insulin secretion. Only in the Met. E, the component 5-hydroxy-4',7-dimethoxyflavone (apigenin-4',7-dimethylether) was detected. Conclusion: It can be concluded that the insulinotropic properties of TP extracts can be attributed to the presence of apigenin existing only in Met. E, but not in Aq. E and fractions. Moreover, certain types of K+ and Ca2+ channels take part in this effect.
Keywords: Teucrium extract, Pancreas, Perfusion, Insulin
Full-Text [PDF 274 kb]      
Type of Study: Full Length | Subject: Related Fields
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirghazanfari S M, Keshavarz M, Nabavizadeh F, Soltani N, Kamalinejad M. The Effect of “Teucrium polium L.” Extracts on Insulin Release from in situ Isolated Perfused Rat Pancreas in a Newly Modified Isolation Method: the Role of Ca2+ and K+ Channels. IBJ. 2010; 14 (4) :178-185
URL: http://ibj.pasteur.ac.ir/article-1-454-en.html


Volume 14, Issue 4 (11-2010) Back to browse issues page
Persian site map - English site map - Created in 0.13 seconds with 37 queries by YEKTAWEB 3647