Iranian
Biomedical Journal
Volume 10, Issue 3 (7-2006)
IBJ 2006, 10(3): 125-131 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Barbarestani M, Ragerdi Kashani I, Etesam F, Shokrgozar M A, Abdolvahabi M A, Haddad P, et al . Evaluation of Human Breast Adenocarcinoma (MCF-7) Cells Proliferation in Co-Culture with Human Adipocytes in Three Dimensional Collagen Gel Matrix: Norepinephrine as a Lipolytic Factor. IBJ 2006; 10 (3) :125-131
URL: http://ibj.pasteur.ac.ir/article-1-366-en.html
URL: http://ibj.pasteur.ac.ir/article-1-366-en.html
Mohammad Barbarestani * 
, Iraj Ragerdi Kashani , Farideh Etesam , Mohammad Ali Shokrgozar , Mir Abbas Abdolvahabi , Peiman Haddad , Mohammad Hossein Noori Mokohi , Mostafa Hosseni
, Iraj Ragerdi Kashani , Farideh Etesam , Mohammad Ali Shokrgozar , Mir Abbas Abdolvahabi , Peiman Haddad , Mohammad Hossein Noori Mokohi , Mostafa Hosseni
Abstract:
Background: Norepinephrine plays a trophic role in the control of cell replication and differentiation in target cells that express adrenergic receptors. Methods: In this study, we have tested the influence of infraphysiological, physiological and supraphysiological concentrations (0.0001 nM, 1 nM, 10000 nM) of human norepinephrine on the proliferation of breast cancer cells (human breast adenocarcinoma [MCF-7]) in co-culture with human adipocytes in three-dimensional collagen gel matrix culture. Cell proliferation and lipolysis rate were measured by 3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) and Oil red O colorimetric assay in second, 7th days of culture experiments. Results: Our results showed a direct correlation between lipolysis rate of adipocytes and proliferation rate of MCF-7 cells. Both physiological and supraphysiological concentrations of human norepinephrine significantly (P<0.05) increased the proliferation of MCF-7 cells synchronously with progress of adipocyte lipolysis. The proliferations of MCF-7 cells were significantly decreased after conversion of adipocytes to fibroblast-like cells by supraphysiological concentration of norepinephrine. There was no statistical difference in lipolysis of adipocytes and proliferation of MCF-7 cells in response to infraphysiological concentration of norepinephrine. Conclusion: These findings indicated that norepinephrine stimulated the proliferation of MCF-7 cells in coculture with human adipocytes as a lipolytic factor and that norepinephrine effect was suppressed by conversion of adipocytes to fibroblast-like cells, suggesting adipocytes as another target for prevention and therapy of breast cancer.
Keywords: Human breast adenocarcinoma (MCF-7) cell, Human adipocyte, Norepinephrine, Proliferation, Lipolysis
Type of Study: Full Length/Original Article |
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
IBJ is a member of Committee on Publication Ethics (COPE)
and follows its guidelines |
The articles of this journal are licensed under
a Creative Commons Attribution-NonDerivatives 4.0 International License. .png) |
 IBJ is owned and published by Pasteur Institute of Iran |