:: Volume 10, Issue 1 (1-2006) ::
IBJ 2006, 10(1): 9-13 Back to browse issues page
Expression and in vitro Characterization of Herpes Simplex Virus 1 (HSV-1) ORF P Protein
Ali Karimi , Mohammad reza Nafisi
Abstract:  
Herpes simplex virus 1 (HSV-1) unspliced 8.3 latency associated transcript (LAT), which located in the long repeat sequences, has been shown to contain at least 16 open reading frames (ORF: A-P). One of these ORF, ORF P, maps almost entirely antisense to HSV-1 neurovirulence gene, ICP34.5. Both ORF P and ICP34.5 are located in the long repeat and are antisense overlapping genes. Therefore, in ORF P deletion mutants, ICP34.5 is also deleted and thus, the characterization of ORF P mutants is almost impossible. An alternative way to analyse its function is to determine those cellular and viral proteins which interact with ORF P. During these experiments, firstly, the expression of full length GlutationeS-transferase (GST)-ORF P fusion protein was optimised and then, using GST-pull down, it was shown that ORF P interacts with a viral and a few cellular proteins in vitro. Conclusively, ORF P might have some functions in HSV-1 replication cycle. 
Keywords: Open reading frames (ORF) P, ICP34.5, Herpes simplex virus 1 (HSV-1)
Full-Text [PDF 152 kb]      
Type of Study: Full Length |
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karimi A, Nafisi M R. Expression and in vitro Characterization of Herpes Simplex Virus 1 (HSV-1) ORF P Protein. IBJ. 2006; 10 (1) :9-13
URL: http://ibj.pasteur.ac.ir/article-1-345-en.html


Volume 10, Issue 1 (1-2006) Back to browse issues page
Persian site map - English site map - Created in 0.18 seconds with 37 queries by YEKTAWEB 3781