Iranian
Biomedical Journal
Volume 22, Issue 6 (11-2018)
IBJ 2018, 22(6): 385-393 |
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Torkashvand F, Mahboudi F, Vossoughi M, Fatemi E, Moosavi Basri S M, Vaziri B. Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line. IBJ 2018; 22 (6) :385-393
URL: http://ibj.pasteur.ac.ir/article-1-2384-en.html
URL: http://ibj.pasteur.ac.ir/article-1-2384-en.html
Fatemeh Torkashvand * 
, Fereidoun Mahboudi , Manouchehr Vossoughi , Elnaz Fatemi , Seyed Masoud Moosavi Basri , Behrouz Vaziri
, Fereidoun Mahboudi , Manouchehr Vossoughi , Elnaz Fatemi , Seyed Masoud Moosavi Basri , Behrouz Vaziri
Abstract:
Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing CHO cell line culture medium were investigated. Results: The glutathione synthesis, the negative regulation of the programmed cell death, proteasomal catabolic process, and the endosomal transport pathway were up-regulated in the group fed with a designed amino acid feed compared to the control group. Conclusion: Our findings could be helpful to identify new targets for metabolic engineering.
Type of Study: Full Length/Original Article |
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