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Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
Kalyani Khanra , Indranil Choudhuri , Nandan Bhattacharyya
Abstract:  
Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. Results: Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis showed a significant (p < 0.001) relationship between pol β mutation and the age of detection. Conclusion: This is a newly reported somatic mutation of pol β in ovarian carcinoma patients from India.
Keywords: DNA polymerase β, Single nucleotide polymorphism, Ovarian cancer
Full-Text [PDF 772 kb]      
Type of Study: Short Communication | Subject: Cancer Biology
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Khanra K, Choudhuri I, Bhattacharyya N. Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples. IBJ. 2018;
URL: http://ibj.pasteur.ac.ir/article-1-2353-en.html


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