:: Volume 21, Issue 5 (9-2017) ::
IBJ 2017, 21(5): 330-337 Back to browse issues page
Synergistic Effects of Arsenic Trioxide and Radiation: Triggering the Intrinsic Pathway of Apoptosis
Kave Moloudi , Ali Neshasteriz , Arshad Hosseini , Nazila Eyvazzadeh , Mehdi Shomali , Samira Eynali , Elahe Mirzaei , Asaad Azarnezhad
Abstract:  

Background: Arsenic trioxide (ATO) has been reported as an effective anti-cancer and a US Food and Drug Administration (FDA) approved drug for treatment of some cancers. The aim of this study is to determine the underlying apoptosis molecular and cellular mechanisms of ATO in the presence or absence of ionizing radiation (IR) in vitro in the glioblastoma multiforme (GBM) cell line, U87MG. Methods: Cells were treated by different concentrations of ATO either in presence or absence of IR. Viability and apoptosis pathway of both treated and control groups were evaluated using MTT assay and the expression analysis of Bax, Bcl-2, and caspase-3 genes, respectively. All treatments were performed on 100-μm diameter spheroids. Results: Results showed a significant reduction in the survival of the cells in all treated groups. As expected, cell survival was much less in combination treatment than treatment with only ATO. Moreover, combination therapy made Bax and caspase-3 up-regulated and Bcl-2 down-regulated. Conclusion: ATO and radiation had a synergistic apoptotic effect on GBM cells by up-regulation of caspase-3 and alteration of the Bax-Bcl-2 balance; therefore, ATO may act as a potential anti-cancer agent against GBM cells through triggering the mitochondrial pathway of apoptosis.

Keywords: Arsenic trioxide, Radiation, Apoptosis, Glioblastoma, Spheroids
Full-Text [PDF 978 kb]      
Type of Study: Full Length | Subject: Cancer Biology
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DOI: 10.18869/acadpub.ibj.21.5.330
PMID: 28459147
PMCID: PMC5548965


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Moloudi K, Neshasteriz A, Hosseini A, Eyvazzadeh N, Shomali M, Eynali S, et al . Synergistic Effects of Arsenic Trioxide and Radiation: Triggering the Intrinsic Pathway of Apoptosis . IBJ. 2017; 21 (5) :330-337
URL: http://ibj.pasteur.ac.ir/article-1-2029-en.html
Volume 21, Issue 5 (9-2017) Back to browse issues page
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