:: Volume 21, Issue 5 (9-2017) ::
IBJ 2017, 21(5): 321-329 Back to browse issues page
Serum Antibodies against Helicobacter pylori Neutrophil Activating Protein in Carriers of IL-4 C-590T Genetic Polymorphism Amplify the Risk of Gastritis and Gastric Cancer
Yeganeh Talebkhan , Mohsen Doozbakhshan , Samaneh Saberi , Maryam Esmaeili , Najmeh Karami , Nazanin Mohajerani , Afshin Abdirad , Mahmoud Eshagh Hosseini , Azin Nahvijou , Mohammad Ali Mohagheghi , Marjan Mohammadi
Abstract:  

Background: Gastric cancer arises, mainly, on an inflammatory background. Helicobacter pylori neutrophil activating (HP-NAP) protein functions as a potent pro-inflammatory mediator.  Similarly, IL-4 plays a critical role in the inflammation pathway, the levels of which are altered by C to T transition at position -590 in its promoter region. Here, we have aimed to assess the risk of gastritis and gastric cancer in the co-presence of these two inflammation modulating mediators. Methods: Gastritis (n=58) and gastric cancer (n=31) patients were evaluated and compared with H. pylori-positive asymptomatic controls (n=46), for serum antibodies against recombinant HP-NAP and IL-4 C-590T single nucleotide polymorphism using immunoblotting and PCR-RFLP, respectively. Multivariable logistic regression, adjusting for age, gender and ethnicity, was used for data analysis. Results: In terms of susceptibility to gastritis, seropositivity to HP-NAP projected a risk impact of 4.62 fold (OR=4.62, 95% CI=1.50-14.22), which when present in IL-4 -590 T carriers augmented the risk up to 9.7 fold (OR=9.70, 95% CI=2.06-45.69). A similar pattern, but of a stronger magnitude, occurred for the risk of gastric cancer, which was estimated at 9.07 fold (OR=9.07, 95% CI=1.99-42.0) for HP-NAP-seropositive subjects and was drastically amplified (OR=33.64, 95% CI=2.06-548.68), when double-positive (HP-NAP seropositive/IL-4 -590 T carrier) subjects were examined against double negatives (HP-NAP seronegative/IL-4 -590 CC). Conclusion: Our preliminary data indicate that serum antibodies against HP-NAP represent a state of risk, which is further exacerbated in IL-4 -590 T carriers. These biomarkers, if validated in larger prospective studies, can be used to screen for gastric cancer susceptibility.

Keywords: Biomarkers, Genetic Polymorphism, Recombinant
Full-Text [PDF 652 kb]      
Type of Study: Full Length | Subject: Molecular Immunology & Vaccines
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DOI: 10.18869/acadpub.ibj.21.5.321
PMID: 27677314
PMCID: PMC5548964


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Talebkhan Y, Doozbakhshan M, Saberi S, Esmaeili M, Karami N, Mohajerani N, et al . Serum Antibodies against Helicobacter pylori Neutrophil Activating Protein in Carriers of IL-4 C-590T Genetic Polymorphism Amplify the Risk of Gastritis and Gastric Cancer. IBJ. 2017; 21 (5) :321-329
URL: http://ibj.pasteur.ac.ir/article-1-1895-en.html
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