:: Volume 20, Issue 5 (11-2016) ::
ibj 2016, 20(5): 273-279 Back to browse issues page
Minocycline Effects on IL-6 Concentration in Macrophage and Microglial Cells in a Rat Model of Neuropathic Pain
Taraneh Moini-Zanjani , Seyed-Nasser Ostad , Farzaneh Labibi , Haleh Ameli , Nariman Mosaffa , Masoumeh Sabetkasaei *
Abstract:  

Background: Evidence indicates that neuropathic pain pathogenesis is not confined to changes in the activity of neuronal systems but involves interactions between neurons, inflammatory immune and immune-like glial cells. Substances released from immune cells during inflammation play an important role in development and maintenance of neuropathic pain. It has been found that minocycline suppresses the development of neuropathic pain. Here, we evaluated the analgesic effect of minocycline in a chronic constriction injury (CCI) model of neuropathic pain in rat and assessed IL-6 concentration from cultured macrophage and microglia cells. Methods: Male Wistar rat (n=6, 150-200 g) were divided into three different groups: 1) CCI+vehicle, 2) sham+vehicle, and 3) CCI+drug. Minocycline (10, 20, and 40 mg/kg) was injected one hour before surgery and continued daily to day 14 post ligation. Von Frey filaments and acetone, as pain behavioral tests, were used for mechanical allodynia and cold allodynia, respectively. Experiments were performed on day 0 (before surgery) and days 1, 3, 5, 7, 10, and 14 post -injury. At day 14, rats were killed and monocyte-derived macrophage from right ventricle and microglia from lumbar part of the spinal cord were isolated and cultured in RPMI and Leibovitz's media, respectively. IL-6 concentration was evaluated in cell culture supernatant after 24 h. Results: Minocycline (10, 20, and 40 mg/kg) attenuated pain behavior, and a decrease in IL-6 concentration was observed in immune cells compared to CCI vehicle-treated animals. Conclusion: Minocycline reduced pain behavior and decreased IL-6 concentration in macrophage and microglial cells. lack;background:white'>drug discovery with H. lepturus scorpion venom.

Keywords: Minocycline, Interleukin-6, Macrophages, Microglia
Full-Text [PDF 527 kb]      
Type of Study: Full Length | Subject: Related Fields
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Moini-Zanjani T, Ostad S, Labibi F, Ameli H, Mosaffa N, Sabetkasaei M. Minocycline Effects on IL-6 Concentration in Macrophage and Microglial Cells in a Rat Model of Neuropathic Pain. ibj. 2016; 20 (5) :273-279
URL: http://ibj.pasteur.ac.ir/article-1-1713-en.html


Volume 20, Issue 5 (11-2016) Back to browse issues page
Persian site map - English site map - Created in 0.2 seconds with 37 queries by YEKTAWEB 3781