:: Volume 20, Issue 4 (9-2016) ::
IBJ 2016, 20(4): 217-222 Back to browse issues page
Improvement of Liver Cell Therapy in Rats by Dietary Stearic Acid
Nasser Hashemi Goradel , Mohammad Ali Eghbal , Masoud Darabi , Leila Roshangar , Maryam Asadi , Nosratollah Zarghami , Mohammad Nouri

Background: Stearic acid is known as a potent anti-inflammatory lipid. This fatty acid has profound and diverse effects on liver metabolism. The aim of this study was to investigate the effect of stearic acid on markers of hepatocyte transplantation in rats with acetaminophen (APAP)-induced liver damage. Methods: Wistar rats were randomly assigned to 10-day treatment. Stearic acid was administered to the rats with APAP-induced liver damage. The isolated liver cells were infused intraperitoneally into rats. Blood samples were obtained to evaluate the changes in the serum liver enzymes, including activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) and the level of serum albumin. To assess the engraftment of infused hepatocytes, rats were euthanized, and the liver DNA was used for PCR using sex-determining region Y (SRY) primers. Results: The levels of AST, ALT and ALP in the serum of rats with APAP-induced liver injury were significantly increased and returned to the levels in control group by day six. The APAP-induced decrease in albumin was significantly improved in rats through cell therapy, when compared with that in the APAP-alone treated rats. SRY PCR analysis showed the presence of the transplanted cells in the liver of transplanted rats. Conclusion: Stearic acid-rich diet in combination with cell therapy accelerates the recovering of hepatic dysfunction in a rat model of liver injury.

Keywords: Stearic acid, Liver failure, Transplantation
Full-Text [PDF 601 kb]      
Type of Study: Full Length | Subject: Medical Biotechnology
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >

XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hashemi Goradel N, Eghbal M A, Darabi M, Roshangar L, Asadi M, Zarghami N et al . Improvement of Liver Cell Therapy in Rats by Dietary Stearic Acid. IBJ. 2016; 20 (4) :217-222
URL: http://ibj.pasteur.ac.ir/article-1-1690-en.html

Volume 20, Issue 4 (9-2016) Back to browse issues page
Persian site map - English site map - Created in 0.128 seconds with 892 queries by yektaweb 3606