:: Volume 20, Issue 2 (4-2016) ::
ibj 2016, 20(2): 68-76 Back to browse issues page
Utilization of Site-Specific Recombination in Biopharmaceutical Production
Maryam Ahmadi , Narges Damavandi , Mohammad Reza Akbari Eidgahi , Fatemeh Davami *

Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and process development, expression level is unpredictable and unstable because of the random location of integration in the genome. Site-specific recombination techniques are capable of producing stable and high producer clonal cells therefore, they are gaining more importance in the biopharmaceutical production. Site-specific recombination methods increase the recombinant protein production by specifically inserting a vector at a locus with specific expression trait. The present review focused on the latest developments in site-specific recombination techniques, their specific features and comparisons.

Keywords: Site-specific recombinase, Gene targeting, CHO cell
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Type of Study: Review Article | Subject: Related Fields
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Ahmadi M, Damavandi N, Akbari Eidgahi M R, Davami F. Utilization of Site-Specific Recombination in Biopharmaceutical Production. ibj. 2016; 20 (2) :68-76
URL: http://ibj.pasteur.ac.ir/article-1-1586-en.html

Volume 20, Issue 2 (4-2016) Back to browse issues page
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