:: Volume 19, Issue 4 (10-2015) ::
IBJ 2015, 19(4): 220-225 Back to browse issues page
Effect of Human Ovarian Tissue Vitrification/Warming on the Expression of Genes Related to Folliculogenesis
Zahra Shams Mofarahe , Marefat Ghaffari Novin , Mina Jafarabadi , Mojdeh Salehnia , Mohsen Noroozian , Nassim Ghorbanmehr
Abstract:  

Background: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vitrification/warming of human ovarian tissue. Methods: Human ovarian tissue samples were collected from five transsexual women. In the laboratory, the ovarian medullary part was removed by a surgical blade, and the cortical tissue was cut into small pieces. Some pieces were vitrified and warmed and the others were considered as non-vitrified group (control). Follicular normality was assessed with morphological observation by a light microscope, and the expression of FIGLA, KIT LIGAND, GDF-9,, and FSHR genes was examined using real-time RT-PCR in both the vitrified and non-vitrified groups. Results: Overall, 85% of the follicles preserved their normal morphologic feature after warming. The percentage of normal follicles and the expression of FIGLA, KIT LIGAND, GDF-9, and FSHR genes were similar in both vitrified and non-vitrified groups (P > 0.05). Conclusion: Vitrification/warming of human ovarian tissue had no remarkable effect on the expression of folliculogenesis-related genes.

Keywords: Vitrification, Gene expression, Humans
Full-Text [PDF 329 kb]      
Type of Study: Full Length | Subject: Related Fields



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Volume 19, Issue 4 (10-2015) Back to browse issues page