Volume 11, Issue 2 (4-2007)                   ibj 2007, 11(2): 125-129 | Back to browse issues page


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Rezvany M R, Kazemi A, Hajifathali A, Kaviani S, Mellstedt H. Analysis of HLA-G Gene Expression in B-Lymphocytes from Chronic Lymphocytic Leukemia Patients. ibj. 2007; 11 (2) :125-129
URL: http://ibj.pasteur.ac.ir/article-1-129-en.html
Abstract:  
The human leukocyte antigen G (HLA-G) molecule exhibits limited tissue distribution, low polymorphism and alternative splicings that generate seven HLA-G isoforms. HLA-G exerts multiple immunoregulatory functions. Recent studies indicate an ectopic up-regulation in tumor cells that may favor their escape from anti-tumor immune responses. This study it is an effort to clarify the presence of HLA-G in B-cell chronic lymphocytic leukemia (B-CLL) patients. Methods: HLA-G mRNA expression was studied in a pilot study in circulating B-CLL and also healthy controls by reverse transcription (RT)-PCR using a set of pan-HLA-gamma primers. Results: RT-PCR was performed on B-cells from 74 B-CLL patients and 12 healthy controls. The data showed HLA-G gene expression in 20% of the B-CLL patients. No expression of HLA-G could be detected in the healthy control group. Conclusion: These data suggest that HLA-G is expressed at the gene level in B cells from B-CLL patients but not in B cells from healthy controls. Further study is required to clarify the role of HLA-gamma as a regulatory factor that could affect immune response in B-CLL patients.
Type of Study: Full Length | Subject: Related Fields

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